ArticleInternational journal of molecular sciences2019
Evaluation of the Major Steps in the Conventional Protocol for the Alkaline Comet Assay.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
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- Protective effects of Paeonia suffruticosa callus extract in skin through anti-inflammation and repair UVB-induced damage.International journal of cosmetic science · 2025Article
- Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Salt and drought stress-mitigating approaches in sugar beet (Beta vulgaris L.) to improve its performance and yield.Planta · 2023Review
- Using the HepaCometChip Assay for Broad-Spectrum DNA Damage Analysis.Current protocols · 2022Article
- A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage.Journal of visualized experiments : JoVE · 2022Article
- Psychiatric Disorders and Genotoxicity Following Primary Metal on Polyethylene Total Hip Arthroplasty and Their Correlation to Cobalt/Chromium Levels.Drug, healthcare and patient safety · 2022Article
- Knockout ofBiomedicines · 2021Article
- Phaseolin, a Protein from the Seed ofNutrients · 2021Article
- Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues.Scientific reports · 2021Article
- Impact of 12-month cryopreservation on endogenous DNA damage in whole blood and isolated mononuclear cells evaluated by the comet assay.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Single cell gel electrophoresis, also known as the comet assay, has become a widespread DNA damage assessment tool due to its sensitivity, adaptability, low cost, ease of use, and reliability. Despite these benefits, this assay has shortcomings, such as long assay running time, the manipulation of multiple slides, individually, through numerous process steps, the challenge of working in a darkened environment, and reportedly considerable inter- and intra-laboratory variation. All researchers typically perform the comet assay based upon a common core approach; however, it appears that some steps in this core have little proven basis, and may exist, partly, out of convenience, or dogma. The aim of this study was to critically re-evaluate key steps in the comet assay, using our laboratory's protocol as a model, firstly to understand the scientific basis for why certain steps in the protocol are performed in a particular manner, and secondly to simplify the assay, and decrease the cost and run time. Here, the shelf life of the lysis and neutralization buffers, the effect of temperature and incubation period during the lysis step, the necessity for drying the slides between the electrophoresis and staining step, and the need to perform the sample workup and electrophoresis steps under subdued light were all evaluated.
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Registered trials
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