ArticleJournal of assisted reproduction and genetics2020
Co-incubation of spermatozoa with human follicular fluid reduces sperm DNA fragmentation by mitigating DNase activity in the seminal plasma.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Sperm DNA Fragmentation: The Concept and Its Use in Assisted Reproduction.Advances in experimental medicine and biology · 2026Review
- Hurdles of Sperm Success: Exploring the Role of DNases.International journal of molecular sciences · 2025Review
- Incubation of semen with human follicular fluid improves the antioxidant status and quality of spermatozoa after freezing-thawing.Reproduction & fertility · 2025Article
- Extracellular vesicles in reproductive medicine: from "animal-led" to "plant-enabled".Frontiers in cell and developmental biology · 2025Review
- Assessing the Efficacy of Acanthoic Acid Isolated fromCurrent issues in molecular biology · 2024Article
- Role of DNase Activity in Human Sperm DNA Fragmentation.Biomolecules · 2024Review
- Effect of bovine and human follicular fluid on semen quality of fresh and frozen-thawed semen in dual purpose Simmental (Fleckvieh) bulls: a new method of adding follicular fluid to bull semen.Iranian journal of veterinary research · 2024Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo examine the effect of co-incubating spermatozoa with human follicular fluid (HFF) on the rate of sperm DNA fragmentation.
methodsThis prospective study used semen (n = 23) and HFF from oocyte donors (n = 23). Liquified semen was divided into four aliquots: (1) neat semen (NEAT), (2) seminal plasma removed and replaced with sperm media (HTF) containing 0% (FF0), (3) 20% (FF20), or (4) 50% (FF50) HFF. Sperm motility and DNA fragmentation (SDF) were assessed following 24 h of incubation at 37 °C. Pro-oxidant capacity of HFF and seminal plasma and the effect of HFF on seminal plasma DNase activity was assessed in a sub-sample of 10 ejaculates.
resultsSperm motility was higher after 3 h of incubation in media that contained HFF compared to the NEAT sample or when sperm was diluted in media without HFF. r-SDF (increase of SDF per time unit) values after 24 h of incubation for NEAT, FF0, FF20 and FF50 were 0.91, 0.69, 0.25 and 0.36, respectively. While pro-oxidant capacity of seminal plasma samples showed large variation (mean: 94.6 colour units; SD 65.4), it was lower and more homogeneous in FF samples (mean: 29.9 colour units; SD: 6.3). Addition of HFF to seminal plasma appeared to inhibit DNase activity.
conclusionWhile differences exist in the pro-oxidant capacity of seminal plasma of patients, sperm DNA integrity was preserved with addition of HFF to sperm media, irrespective of the level of pro-oxidant capacity. DNase activity in the original seminal plasma was abolished after HFF co-incubation.
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