ArticleJournal of mammary gland biology and neoplasia2019
Calcification Microstructure Reflects Breast Tissue Microenvironment.
Article in Journal of mammary gland biology and neoplasia, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- From diagnostic marker-to-therapeutic modality: emerging paradigms in tumor biomineralization.Cancer biology & medicine · 2026Review
- Mechanisms of osteopontin-stabilized amorphous calcium phosphate calcification in benign and pre-malignant breast disease.Scientific reports · 2025Article
- Prostate microcalcification crystallography as a marker of pathology.Scientific reports · 2025Article
- Anisotropy visualisation from X-ray diffraction of biological apatite in mixed phase calcified tissue samples.Scientific reports · 2025Article
- Microcalcifications in breast cancer tissue studied by X-ray absorption, emission, scattering and diffraction.Journal of applied crystallography · 2025Article
- Ultrasonography in granulomatous mastitis: diagnostic differentiation, treatment response, and prognostic value.American journal of translational research · 2025Article
- Characterization of dolomite and calcite microcalcifications in human breast tissue.RSC advances · 2024Article
- Differentiation of histological calcification classifications in breast cancer using ultrashort echo time and chemical shift-encoded imaging MRI.Frontiers in oncology · 2024Article
- Investigating the trends of incidence rates of breast cancer in Southern Iran: a population based survey.BMC women's health · 2023Article
- Whitlockite has a characteristic distribution in mammary microcalcifications and it is not associated with breast cancer.Cancer communications (London, England) · 2023Article
- Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.Analytical methods : advancing methods and applications · 2023Article
- Biomineralogical signatures of breast microcalcifications.Science advances · 2023Article
- The Prospects of Using Structural Phase Analysis of Microcalcifications in Breast Cancer Diagnostics.Diagnostics (Basel, Switzerland) · 2023Article
- Molecular Biology Mechanisms and Emerging Therapeutics of Triple-Negative Breast Cancer.Biologics : targets & therapy · 2023Review
- Breast calcification micromorphology classification.The British journal of radiology · 2022Article
- A multi-modal exploration of heterogeneous physico-chemical properties of DCIS breast microcalcifications.The Analyst · 2022Article
- A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Multiple Pathways for Pathological Calcification in the Human Body.Advanced healthcare materials · 2021Review
- Triple Negative Breast Cancer: A Review of Present and Future Diagnostic Modalities.Medicina (Kaunas, Lithuania) · 2021Review
- Discrimination of Benign and Malignant Lesions in Canine Mammary Tissue Samples Using Raman Spectroscopy: A Pilot Study.Animals : an open access journal from MDPI · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Microcalcifications are important diagnostic indicators of disease in breast tissue. Tissue microenvironments differ in many aspects between normal and cancerous cells, notably extracellular pH and glycolytic respiration. Hydroxyapatite microcalcification microstructure is also found to differ between tissue pathologies, including differential ion substitutions and the presence of additional crystallographic phases. Distinguishing between tissue pathologies at an early stage is essential to improve patient experience and diagnostic accuracy, leading to better disease outcome. This study explores the hypothesis that microenvironment features may become immortalised within calcification crystallite characteristics thus becoming indicators of tissue pathology. In total, 55 breast calcifications incorporating 3 tissue pathologies (benign - B2, ductal carcinoma in-situ - B5a and invasive malignancy - B5b) from archive formalin-fixed paraffin-embedded core needle breast biopsies were analysed using X-ray diffraction. Crystallite size and strain were determined from 548 diffractograms using Williamson-Hall analysis. There was an increased crystallinity of hydroxyapatite with tissue malignancy compared to benign tissue. Coherence length was significantly correlated with pathology grade in all basis crystallographic directions (P < 0.01), with a greater difference between benign and in situ disease compared to in-situ disease and invasive malignancy. Crystallite size and non-uniform strain contributed to peak broadening in all three pathologies. Furthermore, crystallite size and non-uniform strain normal to the basal planes increased significantly with malignancy (P < 0.05). Our findings support the view that tissue microenvironments can influence differing formation mechanisms of hydroxyapatite through acidic precursors, leading to differential substitution of carbonate into the hydroxide and phosphate sites, causing significant changes in crystallite size and non-uniform strain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.