ArticleIranian journal of basic medical sciences2019
Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation.
Article in Iranian journal of basic medical sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- The cryoprotective effect of vitamins on human spermatozoa quality: a systematic review and meta-analysis.Cell and tissue banking · 2022Pooled it
- Vitamin D3 Supplementation Effects on Spermatogram and Oxidative Stress Biomarkers in Asthenozoospermia Infertile Men: a Randomized, Triple-Blind, Placebo-Controlled Clinical Trial.Reproductive sciences (Thousand Oaks, Calif.) · 2022Trial
- The effects of Vitamin D3 supplementation on Spermatogram and endocrine factors in asthenozoospermia infertile men: a randomized, triple blind, placebo-controlled clinical trial.Reproductive biology and endocrinology : RB&E · 2021Trial
- Endocrine-metabolic modulators in sperm biotechnology: cross-species mechanisms and translational priorities.Frontiers in veterinary science · 2026Review
- The effects of supplementation of vitamin D to the egg-yolk extender on cryopreservation of ram semen.Veterinary medicine and science · 2024Article
- High sperm DNA fragmentation: do we have robust evidence to support antioxidants and testicular sperm extraction to improve fertility outcomes? a narrative review.Frontiers in endocrinology · 2023Review
- Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen-Thawed Rooster Sperm?Animals : an open access journal from MDPI · 2022Article
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
- Correlation of Obesity and Serum Vitamin D Levels with Sperm DNA Integrity, Sperm Quality, and Sperm Viability in Normozoospermia Men.Advanced biomedical research · 2022Article
- Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls.Basic and clinical andrology · 2021Article
- Protective effects of vitamin D on learning and memory deficit induced by scopolamine in male rats: the roles of brain-derived neurotrophic factor and oxidative stress.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Inhibitory effect ofExperimental and therapeutic medicine · 2020Article
- Evaluation of Sperm DNA Fragmentation Using Halosperm Technique after the Freezing-Thawing Process in Men: A Study on the Validation of the SCD Protocol.Journal of reproduction & infertilityArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesSperm cryopreservation plays an undeniable role in assisted reproductive technology. However, this process significantly reduces the motility, viability, morphology and nuclear integrity of sperm. Reasons of these changes were oxidative stress and apoptosis. The aim of this study was to evaluate the influence of vitamin D on the survival and integrity of fertile sperm after cryopreservation. MATERIALS AND
methodsSemen sample of 18 males with normal parameters was used. After swimming up, each sample was divided into two parts. 20 µmol vitamin D was added to one part as experimental group and the other part was left untreated as control group. The samples in all groups were frozen for 14 days. Post-thawing, the groups were evaluated for sperm motility, and viability using eosin staining, morphology using the Diff-Quick staining and apoptosis by TUNEL, Annexin-V and caspase-3 activity assay. By using nitrobluetetraxolium test and thiobarbituric acid, the reactive oxygen species (ROS) and lipid peroxidation of sperms were measured, respectively.
resultsIn comparison with control groups, motile and viable sperm concentration was substantially higher in treated groups (
conclusionThe results have shown that the addition of vitamin D to a freezing medium leads to higher quality and function of human sperm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.