ArticlePloS one2019
A scalable culturing system for the marine annelid Platynereis dumerilii.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Long-Term Adverse Effects of Chlorinated Saline Primary Sewage Effluent and Halo-Nitrophenolic Disinfection Byproducts on a Marine Polychaete.Environment & health (Washington, D.C.) · 2026Article
- Evidence of autonomous neural specification for both brain and ventral nerve cord tissue in Annelida.bioRxiv : the preprint server for biology · 2026Article
- Comparative transcriptomics reveal the common anteroposterior molecular blueprint of adult bilaterian guts.PLoS biology · 2026Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid Platynereis dumerilii.Development (Cambridge, England) · 2025Article
- Microalgal biofilm induces larval settlement in the model marine wormRoyal Society open science · 2024Article
- Developmental stage dependent effects of posterior and germline regeneration on sexual maturation in Platynereis dumerilii.Developmental biology · 2024Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelidbioRxiv : the preprint server for biology · 2024Article
- The cost and payout of age on germline regeneration and sexual maturation inbioRxiv : the preprint server for biology · 2024Article
- Article
- A Fast And Versatile Method for Simultaneous HCR, Immunohistochemistry And Edu Labeling (SHInE).Integrative and comparative biology · 2023Article
- The cellular 3D printer of a marine bristle worm-chaetogenesis in Platynereis dumerilii (Audouin & Milne Edwards, 1834) (Annelida).Cell and tissue research · 2023Article
- Marine animal evolutionary developmental biology-Advances through technology development.Evolutionary applications · 2023Article
- Article
- Segment number threshold determines juvenile onset of germline cluster expansion in Platynereis dumerilii.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2022Article
- Studying Annelida Regeneration Using Platynereis dumerilii.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Nereid on the rise: Platynereis as a model system.EvoDevo · 2021Review
- Small-molecule mimicry hunting strategy in the imperial cone snail,Science advances · 2021Article
- An automated aquatic rack system for rearing marine invertebrates.BMC biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Platynereis dumerilii is a marine segmented worm (annelid) with externally fertilized embryos and it can be cultured for the full life cycle in the laboratory. The accessibility of embryos and larvae combined with the breadth of the established molecular and functional techniques has made P. dumerilii an attractive model for studying development, cell lineages, cell type evolution, reproduction, regeneration, the nervous system, and behavior. Traditionally, these worms have been kept in rooms dedicated for their culture. This allows for the regulation of temperature and light cycles, which is critical to synchronizing sexual maturation. However, regulating the conditions of a whole room has limitations, especially if experiments require being able to change culturing conditions. Here we present scalable and flexible culture methods that provide ability to control the environmental conditions, and have a multi-purpose culture space. We provide a closed setup shelving design with proper light conditions necessary for P. dumerilii to mature. We also implemented a standardized method of feeding P. dumerilii cultures with powdered spirulina which relieves the ambiguity associated with using frozen spinach, and helps standardize nutrition conditions across experiments and across different labs. By using these methods, we were able to raise mature P. dumerilii, capable of spawning and producing viable embryos for experimentation and replenishing culture populations. These methods will allow for the further accessibility of P. dumerilii as a model system, and they can be adapted for other aquatic organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.