ArticleMolecular reproduction and development2020
NANOG is required to form the epiblast and maintain pluripotency in the bovine embryo.
Article in Molecular reproduction and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production.The Journal of reproduction and development · 2026Review
- Effect of Small Molecules on Blastocyst Development and Outgrowth Establishment of Bovine Haploid Parthenogenetic Embryos.Animals : an open access journal from MDPI · 2026Article
- Effects of leonurine supplementation during in vitro culture on oxidative stress, cell proliferation, apoptosis, and autophagy in bovine embryos.Scientific reports · 2026Article
- A novel investigation of NANOG and POU5F1 associations in the pluripotent characterization of ES-like and epiblast cells.Scientific reports · 2024Article
- Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification.The Journal of reproduction and development · 2024Article
- In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.Biology of reproduction · 2024Article
- NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.Animal bioscience · 2023Article
- Tauroursodeoxycholic Acid Supplementation in In Vitro Culture of Indicine Bovine Embryos: Molecular and Cellular Effects on the In Vitro Cryotolerance.International journal of molecular sciences · 2023Article
- Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle.Animals : an open access journal from MDPI · 2023Review
- 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress.Animals : an open access journal from MDPI · 2023Article
- Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts.PloS one · 2023Article
- Early Cell Specification in Mammalian Fertilized and Somatic Cell Nuclear Transfer Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts.Cell proliferation · 2022Article
- The Divergent and Conserved Expression Profile of TurtleBiology · 2022Article
- Pluripotent Core in Bovine Embryos: A Review.Animals : an open access journal from MDPI · 2022Review
- Improvements in pig agriculture through gene editing.CABI agriculture and bioscience · 2022Review
- Regulation of NANOG and SOX2 expression by activin A and a canonical WNT agonist in bovine embryonic stem cells and blastocysts.Biology open · 2021Article
- Article
- SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre-implantation embryo development.Cell proliferation · 2021Article
- Pluripotency and Growth Factors in Early Embryonic Development of Mammals: A Comparative Approach.Veterinary sciences · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During preimplantation development, the embryo undergoes two consecutive lineages specifications. The first cell fate decision determines which cells give rise to the trophectoderm (TE) and the inner cell mass (ICM). Subsequently, the ICM differentiates into hypoblast and epiblast, the latter giving rise to the embryo proper. The transcription factors that govern these cell fate decisions have been extensively studied in the mouse, but are still poorly understood in other mammalian species. In the present study, the role of NANOG in the formation of the epiblast and maintenance of pluripotency in the bovine embryo was investigated. Using a CRISPR-Cas9 approach, guide RNAs were designed to target exon 2, resulting in a functional deletion of bovine NANOG at the zygote stage. Disruption of NANOG resulted in the embryos that form a blastocoel and an ICM composed of hypoblast cells. Furthermore, NANOG-null embryos showed lower expression of epiblast cell markers SOX2 and HA2AFZ, and hypoblast marker GATA6; without affecting the expression of TE markers CDX2 and KRT8. Results indicate that NANOG, has no apparent role in segregation or maintenance of the TE, but it is required to derive and maintain the pluripotent epiblast and during the second lineage commitment in the bovine embryo.
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