ArticleFrontiers in chemistry2019
Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters.
Article in Frontiers in chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- The muscle protein synthetic response following corn protein ingestion does not differ from milk protein in healthy, young adults.Amino acids · 2024Trial
- Effect of Hot-Air Drying Temperature on the Physicochemical, Functional, Compositional, and Structural Properties of Catfish (Foods (Basel, Switzerland) · 2026Article
- Food and garden organics (FOGO) compost application improves post-harvest chemical and sensory profiles of conventionally grown tomatoes.NPJ science of food · 2026Article
- Advanced Electrochemical Sensors for Rapid and Sensitive Monitoring of Tryptophan and Tryptamine in Clinical Diagnostics.Biosensors · 2025Review
- The development of a silage-based biorefinery to deliver the maximum nutritional benefit for human consumption from UK grasslands.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Isolation and Quantification of L-Tryptophan fromInsects · 2025Article
- Cultivar selection and processing methods are crucial tools for tailoring the physico-chemical properties and functionality of pea (Current research in food science · 2025Article
- Dietary management for pyridoxine-dependent epilepsy due to α-aminoadipic semialdehyde dehydrogenase deficiency, a follow-on from the international consortium guidelines.JIMD reports · 2024Article
- MoSBiosensors · 2023Article
- Deep Eutectic Solvent Based Reversed-Phase Dispersive Liquid-Liquid Microextraction and High-Performance Liquid Chromatography for the Determination of Free Tryptophan in Cold-Pressed Oils.Molecules (Basel, Switzerland) · 2023Article
- Influence of Chlorella vulgaris on growth, digestibility and gut morphology and microbiota of weaned piglet.Scientific reports · 2022Article
- Recent Advances in Electrochemical and Optical Sensors for Detecting Tryptophan and Melatonin.International journal of nanomedicine · 2021Review
- Rapid Determination of Total Tryptophan in Yoghurt by Ultra High Performance Liquid Chromatography with Fluorescence Detection.Molecules (Basel, Switzerland) · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tryptophan is a key component in many biological processes and an essential amino acid in food and feed materials. Analysis of the tryptophan content in proteins or protein-containing matrices has always been a challenge. We show here that the preparation of samples prior to tryptophan analysis can be significantly simplified, and the time consumption reduced, by using ascorbic acid as antioxidant to eliminate the problem of tryptophan degradation during alkaline hydrolysis. Combined with separation by HPLC and detection by Single Quadrupole Mass Spectrometry, this allows the analytical run time to be reduced to 10 min. The alkaline hydrolysate obtained in the method presented here may be combined with the oxidized hydrolysate obtained when sulfur-containing amino acids are to be measured, thus essentially providing two analyses for the time of one.
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