ArticleFrontiers in microbiology2019
Evaluation of Human Milk Microbiota by 16S rRNA Gene Next-Generation Sequencing (NGS) and Cultivation/MALDI-TOF Mass Spectrometry Identification.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Human milk microbiome as a modulator of the early-life gut-brain axis: mechanisms and translational opportunities for neurodevelopment.Journal of translational medicine · 2026Review
- The human breast milk microbiome: a landscape review of its composition, origins, and impact on infant health.Archives of microbiology · 2026Review
- Human milk microbiota: origins, determinants, and roles in maternal-infant microbial transmission and infant microbiome assembly.Frontiers in microbiology · 2026Review
- New insights into the cultivability of human milk bacteria from ingestion to digestion and implications for their Immunomodulatory properties.Scientific reports · 2025Article
- Changes in Gut Microbiota in Peruvian Cattle Genetic Nucleus by Breed and Correlations with Beef Quality.Veterinary sciences · 2024Article
- Identification and Characterization of Human Breast Milk and Infant Fecal Cultivable Lactobacilli Isolated in Bulgaria: A Pilot Study.Microorganisms · 2024Article
- Article
- Two human milk-like synthetic bacterial communities displayed contrasted impacts on barrier and immune responses in an intestinal quadricellular model.ISME communications · 2024Article
- An optimized culturomics strategy for isolation of human milk microbiota.Frontiers in microbiology · 2024Article
- Questioning the fetal microbiome illustrates pitfalls of low-biomass microbial studies.Nature · 2023Review
- The hidden universe of human milk microbiome: origin, composition, determinants, role, and future perspectives.European journal of pediatrics · 2022Review
- Maternal weight status and the composition of the human milk microbiome: A scoping review.PloS one · 2022Article
- Building Robust Assemblages of Bacteria in the Human Gut in Early Life.Applied and environmental microbiology · 2021Review
- The Species-Level Composition of the FecalMicroorganisms · 2021Article
- Maternal milk microbiota and oligosaccharides contribute to the infant gut microbiota assembly.ISME communications · 2021Article
- Breast Milk: A Meal Worth Having.Frontiers in nutrition · 2021Review
- Comparison of Two Approaches for the Metataxonomic Analysis of the Human Milk Microbiome.Frontiers in cellular and infection microbiology · 2021Article
- The Relationship Between Breast Milk Components and the Infant Gut Microbiota.Frontiers in nutrition · 2021Review
- Distinct Changes Occur in the Human Breast Milk Microbiome Between Early and Established Lactation in Breastfeeding Guatemalan Mothers.Frontiers in microbiology · 2021Article
- Complementary Use of Cultivation and High-Throughput Amplicon Sequencing Reveals High Biodiversity Within Raw Milk Microbiota.Frontiers in microbiology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to characterize human milk microbiota (HMM) with 16S rRNA gene amplicon next-generation sequencing and cultivation/matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) identification approaches. We analyzed 31 human milk samples from healthy Slovenian mothers. To check the accuracy of MALDI-TOF MS identification, several colonies representing most abundant genera and those, which could not be reliably identified by MALDI-TOF, were subjected to Sanger sequencing of their 16S rRNA gene. We showed that cultivation/MALDI-TOF MS was a suitable tool for culture-dependent determination of HMM. With both approaches,
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