ArticleFrontiers in molecular neuroscience2019
Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy.
Article in Frontiers in molecular neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Investigation of the myelin-amyloid interplay in Alzheimer's disease: insights from novel dsCMA imaging in mouse and human brains.Acta neuropathologica communications · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Article
- Birefringence microscopy enables rapid, label-free quantification of myelin debris following induced cortical injury.Neurophotonics · 2025Article
- Accelerating myelin defect detection in neurodegenerative disorders: a human-in-the-loop deep learning approach with birefringence microscopy.Neurophotonics · 2025Article
- A diffractive element-based chromatic confocal thickness measurement device and thickness correction method.iScience · 2025Article
- Label-free and fluorescence imaging of oligodendrocytes and myelin.Npj imaging · 2025Review
- Serum immunoreactivity to neurofilament-medium shows high sensitivity and specificity in patients with Behçet disease.RMD open · 2025Article
- New optical coherence tomography biomarker for diagnosis of acute optic neuritis in multiple sclerosis.Heliyon · 2025Article
- Novel insights into vascular dysfunction in cuprizone-induced demyelination through functional ultrasound imaging.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- Quantitation of the physicochemical properties of myelin using Nile Red fluorescence spectroscopy.Journal of neurochemistry · 2025Article
- C1ql1 expression in oligodendrocyte progenitor cells promotes oligodendrocyte differentiation.The FEBS journal · 2025Article
- Auditory pallial regulation of the social behavior network.Communications biology · 2024Article
- Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Reflective imaging of myelin integrity in the human and mouse central nervous systems.Frontiers in cellular neuroscience · 2024Article
- The phytohormone abscisic acid enhances remyelination in mouse models of multiple sclerosis.Frontiers in immunology · 2024Article
- Development of a Slow-Degrading Polymerized Curcumin Coating for Intracortical Microelectrodes.ACS applied bio materials · 2023Article
- Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2023Review
- Remodelling of myelinated axons and oligodendrocyte differentiation is stimulated by environmental enrichment in the young adult brain.The European journal of neuroscience · 2022Article
- Lipid Metabolism Is Dysregulated in the Motor Cortex White Matter in Amyotrophic Lateral Sclerosis.Metabolites · 2022Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing a high-throughput approach to quantify the extent of myelin integrity in preclinical models of demyelinating diseases will enhance our capacity to identify novel therapies for myelin repair. In light of the technical limitations of electron microscopy and immunohistochemical analyses of myelination, we have utilized a novel imaging technique, spectral confocal reflectance (SCoRe) microscopy. SCoRe takes advantage of the optically reflective properties of compact myelin, allowing the integrity of compact myelin to be quantified over the course of the cuprizone-induced model of central demyelination. We applied SCoRe imaging on fixed frozen brain sections. SCoRe analysis of control mice identified an increase in corpus callosum myelination during the period of cuprizone administration and recovery, suggesting that the normal developmental processes of myelination are ongoing at this time. Importantly, analysis of mice subjected to cuprizone identified a significant reduction in compact myelin in both rostral and caudal corpus callosum compared to age-matched control mice. SCoRe microscopy also allowed the visualization and quantification of the amount of myelin debris in demyelinating lesions. Combining SCoRe imaging with immunohistochemistry, we quantified the amount of myelin debris within IBA-1+ microglia and found that 11% of myelin debris colocalized in microglia irrespective of the callosal regions, with the vast majority of debris outside of microglia. In summary, we have demonstrated that SCoRe microscopy is an effective and powerful tool to perform both quantitative and qualitative analyses of compact myelin integrity in health or after injury
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