ArticleThe Journal of reproduction and development2020
Two novel testis-specific long noncoding RNAs produced by
Article in The Journal of reproduction and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Abnormal Sperm Morphology in Lodpr/lncRNA-HSVIII Knockout Mice with Advancing Age.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- lncRNAInternational journal of molecular sciences · 2025Article
- Spem2, a novel testis-enriched gene, is required for spermiogenesis and fertilization in mice.Cellular and molecular life sciences : CMLS · 2024Article
- Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterus albus.Biology of sex differences · 2023Article
- Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster.BMC genomics · 2022Article
- lncRNA 1700101O22Rik and NONMMUG030480.1 Are Not Essential for Spermatogenesis in Mice.International journal of molecular sciences · 2022Article
- Overexpression of lncRNA-Gm2044 in spermatogonia impairs spermatogenesis in partial seminiferous tubules.Poultry science · 2022Article
- Alterations of mRNA and lncRNA profiles associated with the extracellular matrix and spermatogenesis in goats.Animal bioscience · 2022Article
- Evidence for a functional role of Start, a long noncoding RNA, in mouse spermatocytes.PloS one · 2022Article
- Article
- Revisiting the Characteristics of Testicular Germ Cell Lines GC-1(spg) and GC-2(spd)ts.Molecular biotechnology · 2021Article
- Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.International journal of molecular sciences · 2021Article
- Structural and Functional Characterization of a Testicular Long Non-coding RNAFrontiers in cell and developmental biology · 2021Article
- A Testis-Specific Long Noncoding RNA,Frontiers in endocrinology · 2021Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Testis-specific genes are prone to affect spermatogenesis or sperm fertility, and thus may play pivotal roles in male reproduction. However, whether a gene really affects male reproduction in vivo needs to be confirmed using a gene knock-out (KO) model, a 'gold standard' method. Increasing studies have found that some of the evolutionarily conserved testis-enriched genes are not essential for male fertility. In this study, we report that 1700121C10Rik, a previously uncharacterized gene, is specifically expressed in the testis and produces two long noncoding RNAs (lncRNAs) in mouse: Transcript 1 and Transcript 2. qRT-PCR, northern blotting, and in situ hybridization revealed that expression of both the lncRNAs commenced at the onset of sexual maturity and was predominant in round and elongating spermatids during spermiogenesis. Moreover, we found different subcellular localization of Transcript 1 and Transcript 2 that was predominant in the cytoplasm and nucleus, respectively. 1700121C10Rik-KO mouse model disrupting Transcript 1 and Transcript 2 expression was generated by CRISPR/Cas9 to determine their role in male reproduction. Results showed that 1700121C10Rik-KO male mice were fully fertile with approximately standard testis size, testicular histology, sperm production, sperm morphology, sperm motility, and induction of acrosome reaction. Thus, we conclude that both the testis-specific 1700121C10Rik-produced lncRNAs are dispensable for male fertility in mice under standard laboratory conditions.
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