ArticleBiotechnology reports (Amsterdam, Netherlands)2019
A simple, efficient and rapid screening technique for differentiating nitrile hydratase and nitrilase producing bacteria.
Article in Biotechnology reports (Amsterdam, Netherlands), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 11 citations in OpenAlex.
- Promiscuous nitrilase from Bacillus subtilis for herbicide degradation and plant growth promotion.Scientific reports · 2026Article
- Novel Haloalkaliphilic Nitrile-Degrading Bacteria From Soda Lake Soil of Rift Valley Kenya.TheScientificWorldJournal · 2025Article
- Response surface methodology: An effective optimization strategy for enhanced production of nitrile hydratase (NHase) byHeliyon · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Nitrile hydrolyzing enzymes catalyze the hydration of nitrile compounds to corresponding amides and acids. Bacteria, isolated from soil samples were screened for nitrile hydrolyzing enzymes by simple dye based 96 well plate and nesslerization method. Bromothymol blue was used as an indicator for the detection of amides and acids based on colour change of the indicator dye from blue to dark green or yellow. The screening assay also differentiates between nitrile hydratase (NHase) and nitrilase producing bacteria. Among the 108 bacterial strains screened for enzyme activity, six strains were positive for NHase activity and eleven strains were positive for nitrilase activity based on their ability to degrade acrylonitrile into products. The strain showing maximum NHase activity in quantitative assay was identified as
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