ArticlePlant methods2019
An efficient system composed of maize protoplast transfection and HPLC-MS for studying the biosynthesis and regulation of maize benzoxazinoids.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Plant Volatile Methyl Salicylate Primes Wheat Defense Against the Grain Aphid by Altering the Synthesis of Defense Metabolites.Plant, cell & environment · 2026Article
- TargetGAN: A generative AI framework for the design of plant core promoters with targeted activity.Plant communications · 2026Article
- A maize protoplast transfection system for studying the biosynthesis of volatile terpenoids.Crop health · 2026Article
- From Natural Discovery to AI-Guided Design: A Curated Collection of Compact Enhancers for Crop Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Isolation and Transfection of Protoplasts From Maize Mesophyll Cells.Bio-protocol · 2026Article
- Rational design of promoter editing confers multipathogen resistance in rice.Genome biology · 2025Article
- Rapid assessment of CRISPR gRNAs with optimized protoplast transformation in Maize.Plant cell reports · 2025Article
- The fall armyworm converts maize endophytes into its own probiotics to detoxify benzoxazinoids and promote caterpillar growth.Microbiome · 2024Article
- A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine-tune the high-density planting adaption in maize.Plant biotechnology journal · 2024Article
- Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli.Genes · 2024Review
- ZmARF1 positively regulates low phosphorus stress tolerance via modulating lateral root development in maize.PLoS genetics · 2024Article
- Production of secondary metabolites using tissue culture-based biotechnological applications.Frontiers in plant science · 2023Review
- Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- ZmmiR169q/ZmNF-YA8 is a module that homeostatically regulates primary root growth and salt tolerance in maize.Frontiers in plant science · 2023Article
- The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat.Journal of experimental botany · 2022Article
- New insights into the transcriptional regulation of benzoxazinoid biosynthesis in wheat.Journal of experimental botany · 2022Article
- Effects of Nitrogen Supply on Induced Defense in Maize (International journal of molecular sciences · 2022Article
- Optimization of an Efficient Protoplast Transformation System for Transient Expression Analysis Using Leaves ofPlants (Basel, Switzerland) · 2022Article
- miR169q and NUCLEAR FACTOR YA8 enhance salt tolerance by activating PEROXIDASE1 expression in response to ROS.Plant physiology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInsect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known about how benzoxazinoids are regulated in maize, partly due to lack of mutant resources and recalcitrance to genetic transformation. Transient systems based on mesophyll- or cultured cell-derived protoplasts have been exploited in several plant species and have become a powerful tool for rapid or high-throughput assays of gene functions. Nevertheless, these systems have not been exploited to study the regulation of secondary metabolites.
resultsA protocol for isolation of protoplasts from etiolated maize seedlings and efficient transfection was optimized. Furthermore, a 10-min-run-time and highly sensitive HPLC-MS method was established to rapidly detect and quantify maize benzoxazinoids. Coupling maize protoplast transfection and HPLC-MS, we screened a few genes potentially regulating benzoxazinoid biosynthesis using overexpression or silencing by artificial microRNA technology.
conclusionsCombining the power of maize protoplast transfection and HPLC-MS analysis, this method allows rapid screening for the regulatory and biosynthetic genes of maize benzoxazinoids in protoplasts, before the candidates are selected for in planta functional analyses. This method can also be applied to study the biosynthesis and regulation of other secondary metabolites in maize and secondary metabolites in other plant species, including those not amenable to transformation.
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