ArticleJournal of applied crystallography2019
Successful sample preparation for serial crystallography experiments.
Article in Journal of applied crystallography, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Review
- Protein crystallization and structure determination at room temperature in the CrystalChip.FEBS open bio · 2025Article
- CHiMP: deep-learning tools trained on protein crystallization micrographs to enable automation of experiments.Acta crystallographica. Section D, Structural biology · 2024Article
- Article
- In situ serial crystallography facilitates 96-well plate structural analysis at low symmetry.IUCrJ · 2024Article
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- Efficient in situ screening of and data collection from microcrystals in crystallization plates.Acta crystallographica. Section D, Structural biology · 2024Article
- A redox switch allows binding of Fe(II) and Fe(III) ions in the cyanobacterial iron-binding protein FutA fromProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Guide to serial synchrotron crystallography.Current research in structural biology · 2024Review
- Growing Crystals for X-ray Free-Electron Laser Structural Studies of Biomolecules and Their Complexes.International journal of molecular sciences · 2023Article
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- JINXED: just in time crystallization for easy structure determination of biological macromolecules.IUCrJ · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Room temperature crystallography and X-ray spectroscopy of metalloenzymes.Methods in enzymology · 2023Article
- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.Methods in molecular biology (Clifton, N.J.) · 2023Article
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Authors and funding
11 authors at 6 institutions in 2 countries.
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Abstract
Serial crystallography, at both synchrotron and X-ray free-electron laser light sources, is becoming increasingly popular. However, the tools in the majority of crystallization laboratories are focused on producing large single crystals by vapour diffusion that fit the cryo-cooled paradigm of modern synchrotron crystallography. This paper presents several case studies and some ideas and strategies on how to perform the conversion from a single crystal grown by vapour diffusion to the many thousands of micro-crystals required for modern serial crystallography grown by batch crystallization. These case studies aim to show (i) how vapour diffusion conditions can be converted into batch by optimizing the length of time crystals take to appear; (ii) how an understanding of the crystallization phase diagram can act as a guide when designing batch crystallization protocols; and (iii) an accessible methodology when attempting to scale batch conditions to larger volumes. These methods are needed to minimize the sample preparation gap between standard rotation crystallography and dedicated serial laboratories, ultimately making serial crystallography more accessible to all crystallographers.
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