ArticleNature communications2019
Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed.
- Genetic evaluation of CRISPR-Cas9 off-target effects from deleterious mutations on Drosophila male single X chromosome.Functional & integrative genomics · 2025Article
- Genome-wide Genetic Mutations Accumulated in Pigs Genome-edited for Xenotransplantation and Their Filial Generation.Genomics, proteomics & bioinformatics · 2025Article
- VDGE: a data repository of variation database for gene-edited animals across multiple species.Nucleic acids research · 2025Article
- Characterization and distribution of de novo mutations in the zebra finch.Communications biology · 2024Article
- Modeling SHANK3-associated autism spectrum disorder in Beagle dogs via CRISPR/Cas9 gene editing.Molecular psychiatry · 2023Article
- The history, use, and challenges of therapeutic somatic cell and germline gene editing.Fertility and sterility · 2023Review
- DANGER analysis: risk-averse on/off-target assessment for CRISPR editing without a reference genome.Bioinformatics advances · 2023Article
- VCFshiny: an R/Shiny application for interactively analyzing and visualizing genetic variants.Bioinformatics advances · 2023Article
- Whole Genome Sequencing Analysis of Effects of CRISPR/Cas9 inJournal of fungi (Basel, Switzerland) · 2022Article
- CRISPR/Cas9 genome editing to create nonhuman primate models for studying stem cell therapies for HIV infection.Retrovirology · 2022Review
- Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis.Science advances · 2022Article
- Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Modeling genetic diseases in nonhuman primates through embryonic and germline modification: Considerations and challenges.Science translational medicine · 2022Review
- CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations.Nature communications · 2022Article
- Application of CRISPR/Cas9 System in Establishing Large Animal Models.Frontiers in cell and developmental biology · 2022Review
- Whole genome sequencing ofFrontiers in genome editing · 2022Article
- Atomic-scale insights into allosteric inhibition and evolutional rescue mechanism ofComputational and structural biotechnology journal · 2021Article
- Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.Scientific reports · 2020Article
- Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity.Genome biology · 2020Article
- The dawn of non-human primate models for neurodevelopmental disorders.Current opinion in genetics & development · 2020Review
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequencing of Cas9-treated rhesus monkeys. We only find a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected off-target mutations (OTMs) were detected. Furthermore, the long-read sequencing data does not detect large structural variants in the target region.
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