ArticleGenes & genomics2020
De novo transcriptome analysis of stressed blood clam (Anadara broughtonii) and identification of genes associated with hemoglobin.
Article in Genes & genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Revealing the Impact of Polystyrene Nanoplastics on Gill Tissues of the Intertidal Clam, Gafrarium Divaricatum (Gmelin, 1791) using Transcriptomics Approach.Marine biotechnology (New York, N.Y.) · 2026Article
- A review of the endangered mollusks transcriptome under the threatened species initiative of Korea.Genes & genomics · 2023Review
- The Moonlighting Function of Soybean Disordered Methyl-CpG-Binding Domain 10c Protein.International journal of molecular sciences · 2023Article
- Exploration of anti-stress mechanisms in high temperature exposed juvenile golden cuttlefish (Frontiers in physiology · 2023Article
- Exploration of immune response mechanisms in cadmium and copper co-exposed juvenile golden cuttlefish (Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlood clam (Anadara broughtonii) is a commercially important marine bivalve characterised by the red blood. Recently, the clams have been subjected to severe resource recession. Multiple environmental stressors are indispensable for the recession.
objectiveWe aimed to investigate the transcriptome information of blood clam under environmental stressors.
methodsPaired-end Illumina HiSeq™ 2500 sequencing technology was employed for cDNA library construction and Illumina sequencing. Several public databases were introduced for gene annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used for pathways analyses. The Open Reading Frame of annotated hemoglobin (Hb) was predicted and validated by DNAMAN 6.0 and NCBI BLASTx, respectively.
resultsA total of 242,919 transcripts were generated, 116,264 unigenes were subsequently assembled with an average length of 747 base pairs, and 33,776 unigenes were successfully annotated. Gene Ontology (GO) categories indicated that the terms of cellular processes, metabolic processes, cell, cell part, binding, and catalytic activity were dominant. KEGG pathway analyses suggested ribosome, oxytocin, focal adhesion, Ras, and PI3K-Akt were the largest signaling pathway groups, and many presented pathways (Ras, Rap1, and MAPK, etc.) were related to apoptosis, immune and stress response. In addition, a total of 19,306 potential simple sequence repeats (SSRs) were detected in 15,852 sequences. Six hemoglobin-related genes with complete conserved domain sequences were identified and 3 of them were predicted as HbI, HbIIα, and HbIIβ.
conclusionThis study provides transcriptome responses to multiple environmental stressors in blood clams and would provide interesting hints for further studies.
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