ArticleJournal, genetic engineering & biotechnology2019
Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt.
Article in Journal, genetic engineering & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Performance comparison of gel and capillary electrophoresis-based microsatellite genotyping strategies in a population research and kinship testing framework.BMC research notes · 2021Article
- Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes.Journal, genetic engineering & biotechnology · 2021Article
- EgyGene GelAnalyzer4: a powerful image analysis software for one-dimensional gel electrophoresis.Journal, genetic engineering & biotechnology · 2021Article
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1 author at 1 institution in 1 country.
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Abstract
backgroundSilybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt.
resultsThe electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples.
conclusionThe obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.
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