ArticleProceedings of the National Academy of Sciences of the United States of America2019
Engineering geometrical 3-dimensional untethered in vitro neural tissue mimic.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Three-dimensional microelectrode arrays forBiomedical engineering letters · 2026Review
- Cell-generated mechanical forces play a role in epileptogenesis after injury.Experimental neurology · 2025Article
- Optogenetic neuromuscular actuation of a miniature electronic biohybrid robot.Science robotics · 2025Article
- The Morphological, Behavioral, and Transcriptomic Life Cycle of Anthrobots.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Basal Xenobot transcriptomics reveals changes and novel control modality in cells freed from organismal influence.Communications biology · 2025Article
- Actuating Extracellular Matrices Decouple the Mechanical and Biochemical Effects of Muscle Contraction on Motor Neurons.Advanced healthcare materials · 2025Article
- Advancing biohybrid robotics: Innovations in contraction models, control techniques, and applications.Biophysics reviews · 2025Review
- Mind In Vitro Platforms: Versatile, Scalable, Robust, and Open Solutions to Interfacing with Living Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- 3D bioprinting of human neural tissues with functional connectivity.Cell stem cell · 2024Article
- Engineering multicellular living systems-a Keystone Symposia report.Annals of the New York Academy of Sciences · 2022Article
- High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors.Communications biology · 2022Article
- Principles for the design of multicellular engineered living systems.APL bioengineering · 2022Article
- 3D Bioprinting of Engineered Tissue Flaps with Hierarchical Vessel Networks (VesselNet) for Direct Host-To-Implant Perfusion.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Advanced Optogenetic-Based Biosensing and Related Biomaterials.Materials (Basel, Switzerland) · 2021Review
- Compliant 3D frameworks instrumented with strain sensors for characterization of millimeter-scale engineered muscle tissues.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Formation of tissue models in 3 dimensions is more effective in recapitulating structure and function compared to their 2-dimensional (2D) counterparts. Formation of 3D engineered tissue to control shape and size can have important implications in biomedical research and in engineering applications such as biological soft robotics. While neural spheroids routinely are created during differentiation processes, further geometric control of in vitro neural models has not been demonstrated. Here, we present an approach to form functional in vitro neural tissue mimic (NTM) of different shapes using stem cells, a fibrin matrix, and 3D printed molds. We used murine-derived embryonic stem cells for optimizing cell-seeding protocols, characterization of the resulting internal structure of the construct, and remodeling of the extracellular matrix, as well as validation of electrophysiological activity. Then, we used these findings to biofabricate these constructs using neurons derived from human embryonic stem cells. This method can provide a large degree of design flexibility for development of in vitro functional neural tissue models of varying forms for therapeutic biomedical research, drug discovery, and disease modeling, and engineering applications.
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