ArticleBMC oral health2019
Comparing dental plaque microbiome diversity of extrinsic black stain in the primary dentition using Illumina MiSeq sequencing technique.
Article in BMC oral health, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Chromogenic bacterial staining of teeth: a scoping review.BMC oral health · 2025Pooled it
- Postbiotic gel relieves clinical symptoms of bacterial vaginitis by regulating the vaginal microbiota.Frontiers in cellular and infection microbiology · 2023Trial
- Supragingival Biomarker flora of Children With and Without Cariogenic Disease and Black Stains, Aged 3 to 6 Years.International dental journal · 2026Article
- Microbial Characteristics of the Extrinsic Black Stain in Primary Dentition.International dental journal · 2026Article
- Mapping the oral microbiome in children with oral diseases using next-generation sequencing: a systematic review.Journal of oral microbiology · 2026Review
- Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review.Journal of dental sciences · 2024Review
- Effect of A Fluoride Toothpaste Containing Enzymes and Salivary Proteins on Periodontal Pathogens in Subjects with Black Stain: A Pilot Study.European journal of dentistry · 2024Article
- Genomic Diversity amongMicroorganisms · 2023Article
- Characterization of the oral microbiome and gut microbiome of dental caries and extrinsic black stain in preschool children.Frontiers in microbiology · 2023Article
- Epidemiological and Microbiome Characterization of Black Tooth Stain in Preschool Children.Frontiers in pediatrics · 2022Article
- Dental black plaque: metagenomic characterization and comparative analysis with white-plaque.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExtrinsic black stain (EBS) is characterized by discrete dark dots or lines on the tooth surface. The relationship between EBS and oral microbiota in children remains elusive. The aim of this study was to compare dental plaque microbiome in EBS children with that in EBS-free children in the primary dentition.
methodsThe Illumina MiSeq sequencing technique was utilized in the cross-sectional pilot study to investigate the diversity and composition of the supragingival plaque microbiota from 10 EBS-positive and 10 EBS-free children. The results were analysed with nonparametric Mann-Whitney U test, Pearson Chi-Square test, Fisher's Exact test and one-way ANOVA tests.
resultsWe identified 13 different phyla, 22 classes, 33 orders, 54 families, 105 genera, and 227 species from a total of 52,646 high-quality sequences. Between two groups, no statistical differences were observed in the estimators of community richness and diversity at 97% similarity, as well as in the Unweighted Unifrac principal co-ordinates analysis (PCoA). At the species level, higher level of relative abundance of Actinomyces naeslundii and lower level of relative abundance of a species belonging to Candidate_division_TM7 was observed in dental plaque of EBS-positive subjects, compared to dental plaque of EBS-free subjects (P < 0.05). This indicated that some species might be involved in the EBS process.
conclusionChanges in dental plaque microbiota is possibly relevant to the process of EBS in the primary dentition.
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