ArticleBMC microbiology2019
Prevalence of fluoroquinolone resistance and mutations in the gyrA, parC and parE genes of Riemerella anatipestifer isolated from ducks in China.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 22 citations in OpenAlex.
- Phylogenetic analysis and susceptibility to antibiotics and phytogenics ofJournal of veterinary research · 2026Article
- Study on Antimicrobial Resistance and Molecular Characteristics ofAnimals : an open access journal from MDPI · 2026Article
- Emerging resistance in genital mycoplasmas: 6-year trends of Ureaplasma spp. and Mycoplasma hominis infections in Eastern China.BMC infectious diseases · 2025Article
- Genomic analysis reveals two dominant strains ofApplied and environmental microbiology · 2025Article
- Quinolone resistance inVeterinary world · 2025Article
- Antimicrobial Susceptibility Profiles ofAntibiotics (Basel, Switzerland) · 2025Article
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- Evolution of Resistance against Ciprofloxacin, Tobramycin, and Trimethoprim/Sulfamethoxazole in the Environmental Opportunistic PathogenAntibiotics (Basel, Switzerland) · 2024Article
- Strain typing and antimicrobial susceptibility ofHeliyon · 2024Article
- Genome-based assessment of antimicrobial resistance reveals the lineage specificity of resistance and resistance gene profiles inMicrobiology spectrum · 2024Article
- Chlorogenic acid attenuates tet (X)-mediated doxycycline resistance ofFrontiers in veterinary science · 2024Article
- Enrofloxacin-The Ruthless Killer of Eukaryotic Cells or the Last Hope in the Fight against Bacterial Infections?International journal of molecular sciences · 2022Review
- Assessment of animal diseases caused by bacteria resistant to antimicrobials: Poultry.EFSA journal. European Food Safety Authority · 2021Article
- Antimicrobial Resistance in ClinicalAntimicrobial agents and chemotherapy · 2020Article
- Predominance of III/ST19 and Ib/ST10 Lineages With High Multidrug Resistance in Fluoroquinolone-Resistant Group BFrontiers in microbiology · 2020Article
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Authors and funding
18 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRiemerella anatipestifer is one of the most serious infectious disease-causing pathogens in the duck industry. Drug administration is an important method for prevention and treatment of infection in duck production, leading to widespread drug resistance in R. anatipestifer.
methodsFor a total of 162 isolates of R. anatipestifer, the MICs were determined for a quinolone antimicrobial agent, namely, nalidixic acid, and three fluoroquinolones, namely, ciprofloxacin, enrofloxacin and ofloxacin. The gyrA, parC, and parE gene fragments were amplified by PCR to identify the mutation sites in these strains. Site-directed mutants with mutations that were detected at a high frequency in vivo were constructed (hereafter referred to as site-directed in vivo mutants), and the MICs of these four drugs for these strains were determined.
resultsIn total, 100, 97.8, 99.3 and 97.8% of the 137 R. anatipestifer strains isolated between 2013 and 2018 showed resistance to nalidixic acid, ciprofloxacin, enrofloxacin, and ofloxacin, respectively. The high-frequency mutation sites were detected in a total of 162 R. anatipestifer strains, such as Ser83Ile and Ser83Arg, which are two types of substitution mutations of amino acid 83 in GyrA; Val799Ala and Ile811Val in ParC; and Val357Ile, His358Tyr, and Arg541Lys in ParE. MIC analysis results for the site-directed in vivo mutants showed that the strains with only the Ser83Ile mutation in GyrA exhibited an 8-16-fold increase in MIC values, and all mutants showed resistance to ampicillin and ceftiofur.
conclusionsThe resistance of R. anatipestifer to quinolone agents is a serious problem. Amino acid 83 in GyrA is the major target mutation site for the fluoroquinolone resistance mechanism of R. anatipestifer.
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