ArticleMicromachines2019
Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device.
Article in Micromachines, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Probing the Dynamic Strength of Biomolecular Interactions with Single-Cell Centrifugation.ACS central science · 2025Article
- High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level.bioRxiv : the preprint server for biology · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.Scientific reports · 2025Article
- High sensitivity tapered fiber refractive index biosensor using hollow gold nanoparticles.Scientific reports · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.bioRxiv : the preprint server for biology · 2024Article
- Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols.The journal of physical chemistry. B · 2024Article
- Three-dimensional topology-based analysis segments volumetric and spatiotemporal fluorescence microscopy.Biological imaging · 2024Article
- Tumor-on-chip platforms for breast cancer continuum concept modeling.Frontiers in bioengineering and biotechnology · 2024Review
- Easy and Accessible Workflow for Label-Free Single-Cell Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
- Transcriptional Response in Human Jurkat T Lymphocytes to a near Physiological Hypergravity Environment and to One Common in Routine Cell Culture Protocols.International journal of molecular sciences · 2023Article
- Characterizing cellular membrane partitioning of DMSO using low-temperature Raman spectroscopy.Frontiers in molecular biosciences · 2023Article
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Rapid Customization and Manipulation Mechanism of Micro-Droplet Chip for 3D Cell Culture.Micromachines · 2022Article
- Lack of Nck1 protein and Nck-CD3 interaction caused the increment of lipid content in Jurkat T cells.BMC molecular and cell biology · 2022Article
- Poroelastic osmoregulation of living cell volume.iScience · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Jurkat cell is an immortalized line of human acute lymphocyte leukemia cells that is widely used in the study of adoptive cell therapy, a novel treatment of several advanced forms of cancer. The ability to transport water and solutes across the cell membrane under different temperatures is an important factor for deciding the specific protocol for cryopreservation of the Jurkat cell. In this study we propose a comprehensive process for determination of membrane transport properties of Jurkat cell. using a novel microfluidic controlled single cell-trapping system. The osmotic behavior of an individual Jurkat cell to water and dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA), under constant temperature, was recorded under a microscope utilizing the modified microfluidic system. The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time. The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane permeability to water and DMSO at room temperature (22 °C). This model and the calculated parameters can help scientists optimize the cryopreservation protocol for any cell type with optimal cryoprotective agents and cooling rate for future experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.