ArticlePloS one2019
Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Impact of feeding diets without mineral P supplement on the immune system of two laying hen strains.Poultry science · 2026Article
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- Effects of dietary myo-inositol supplementation on the expression of fibroblast growth factor 23 (FGF23) and αKlotho in two commercial laying hen strains.Poultry science · 2026Article
- Evaluating the Immunogenicity and Protective Efficacy of a Novel Vaccine Candidate AgainstVaccines · 2026Article
- Interleukin 10 controls the balance between tolerance, pathogen elimination, and immunopathology in birds.eLife · 2025Article
- The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.Communications biology · 2024Article
- Integration of Image Pattern Recognition and Photon Sensor for Analyzing Cytokine Gene Expression Using πCode MicroDisc.Biosensors · 2024Article
- FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155.Veterinary research · 2024Article
- Chicken intestinal organoids: a novel method to measure the mode of action of feed additives.Frontiers in immunology · 2024Article
- CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy.PLoS pathogens · 2023Article
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- Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility.Veterinary research · 2023Article
- A decline in avian cytokine expression with age revealed by commercially available cytokine array.Frontiers in physiology · 2023Article
- Disentangling the innate immune responses of intestinal epithelial cells and lamina propria cells toFrontiers in microbiology · 2023Article
- The molecular basis of differential host responses to avian influenza viruses in avian species with differing susceptibility.Frontiers in cellular and infection microbiology · 2023Article
- Evidence for Different Virulence Determinants and Host Response after Infection of Turkeys and Chickens with Highly Pathogenic H7N1 Avian Influenza Virus.Journal of virology · 2022Article
- Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer.Veterinary research · 2021Article
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Authors and funding
7 authors.
Funding
Abstract
To address the need for sensitive high-throughput assays to analyse avian innate and adaptive immune responses, we developed and validated a highly multiplexed qPCR 96.96 Fluidigm Dynamic Array to analyse the transcription of chicken immune-related genes. This microfluidic system permits the simultaneous analysis of expression of 96 transcripts in 96 samples in 6 nanolitre reactions and the 9,216 reactions are ready for interpretation immediately. A panel of 89 genes was selected from an RNA-seq analysis of the transcriptional response of chicken macrophages, dendritic cells and heterophils to agonists of innate immunity and from published transcriptome data. Assays were confirmed to be highly specific by amplicon sequencing and melting curve analysis and the reverse transcription and preamplification steps were optimised. The array was applied to RNA of various tissues from a commercial line of broiler chickens housed at two different levels of biosecurity. Gut-associated lymphoid tissues, bursa, spleen and peripheral blood leukocytes were isolated and transcript levels for immune-related genes were defined. The results identified blood cells as a potentially reliable indicator of immune responses among all the tissues tested with the highest number of genes significantly differentially transcribed between birds housed under varying biosecurity levels. Conventional qPCR analysis of three differentially transcribed genes confirmed the results from the multiplex qPCR array. A highly multiplexed qPCR-based platform for evaluation of chicken immune responses has been optimised and validated using samples from commercial chickens. Apart from applications in selective breeding programmes, the array could be used to analyse the complex interplay between the avian immune system and pathogens by including pathogen-specific probes, to screen vaccine responses, and as a predictive tool for immune robustness.
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