Evidence map›Paper›PMID 31792295›Full record

ArticleScientific reports2019

Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a.

Kunwadee Palasin, Tamayo Uechi, Maki Yoshihama, Naparee Srisowanna, Narantsog Choijookhuu, Yoshitaka Hishikawa, Naoya Kenmochi, Wilaiwan Chotigeat

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Research Progress of Ribosomal Proteins in Reproductive Development.International journal of molecular sciences · 2024
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Using the Zebrafish as a Genetic Model to Study Erythropoiesis.International journal of molecular sciences · 2021
    Review
  10. Role ofInternational journal of molecular sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Kunwadee PalasinDepartment of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand.
Tamayo UechiFrontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan.
Maki YoshihamaFrontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan.
Naparee SrisowannaDepartment of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan.
Narantsog ChoijookhuuDepartment of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan.
Yoshitaka HishikawaDepartment of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan.
Naoya KenmochiFrontier Science Research Centre, University of Miyazaki, Kiyotake, Miyazaki, 889-1692, Japan. kenmochi@med.miyazaki-u.ac.jp.
Wilaiwan ChotigeatDepartment of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand. wilaiwan58@hotmail.com.
University of Miyazaki · JPPrince of Songkla University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, to investigate the secondary function of Rpl10a in zebrafish development, morpholino antisense oligonucleotides (MOs) were used to knock down the zebrafish ribosomal protein L10a (rpl10a). At 25 hpf (hours post-fertilization), embryos injected with the rpl10a MO showed an abnormal morphology, including short bodies, curved tails, and small yolk sac extensions. We observed pigment reductions, edema, larger yolk sacs, smaller eyes and smaller yolk sac extensions at 50 hpf. In addition, reductions in the expression of primordial germ cell (PGC) marker genes (nanos1 and vasa) were observed in rpl10a knockdown embryos. A rescue experiment using a rpl10a mRNA co-injection showed the recovery of the morphology and red blood cell production similar to wild-type. Moreover, the CRISPR-Cas9 system was used to edit the sequence of rpl10a exon 5, resulting in a homozygous 5-bp deletion in the zebrafish genome. The mutant embryos displayed a morphology similar to that of the knockdown animals. Furthermore, the loss of rpl10a function led to reduced expression of gata1, hbae3, and hbbe1 (erythroid synthesis) and increased tp53 expression. Overall, the results suggested that Rpl10a deficiency caused delays in embryonic development, as well as apoptosis and anemia, in zebrafish.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsCRISPR-Cas SystemsDEAD-box RNA HelicasesEmbryo, NonmammalianErythropoiesisGATA1 Transcription FactorGene Knockdown TechniquesGene Knockout TechniquesGerm CellsHemoglobinsOligonucleotides, AntisenseRNA-Binding ProteinsTumor Suppressor Protein p53ZebrafishZebrafish Proteinsddx4 protein, zebrafishDEAD-box RNA Helicasesgata1a protein, zebrafishGATA1 Transcription FactorHemoglobinsnanos3 protein, zebrafishOligonucleotides, AntisenseRNA-Binding ProteinsRpl10a protein, zebrafishtp53 protein, zebrafishTumor Suppressor Protein p53Zebrafish Proteins

Identifiers

PMID31792295
PMCPMC6889351
OpenAlexW2989644817

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.