Evidence map›Paper›PMID 31791392›Full record

ArticleClinical epigenetics2019

Changes in DNA methylation from pre- to post-adolescence are associated with pubertal exposures.

Luhang Han, Hongmei Zhang, Akhilesh Kaushal, Faisal I Rezwan, Latha Kadalayil, Wilfried Karmaus, A John Henderson, Caroline L Relton, Susan Ring, S Hasan Arshad and 2 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical epigenetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 5 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 5 syntheses or guidelines pooled it, 71 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 2 countries.

Luhang HanDepartment of Mathematical Sciences, University of Memphis, Memphis, TN, 38152, USA.
Hongmei ZhangDivision of Epidemiology, Biostatistics, and Environmental Health, University of Memphis, Memphis, TN, 38152, USA. hzhang6@memphis.edu.ORCID 0000-0003-3557-0364
Akhilesh KaushalSchool of Medicine, Emory University, Atlanta, GA, 30322, USA.
Faisal I RezwanSchool of Water, Energy and Environment, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK.
Latha KadalayilHuman Development and Health, Faculty of Medicine, University of Southampton, Southampton, SO17 1BJ, UK.
Wilfried KarmausDivision of Epidemiology, Biostatistics, and Environmental Health, University of Memphis, Memphis, TN, 38152, USA.
A John HendersonPopulation Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS8 1QU, UK.
Caroline L ReltonPopulation Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS8 1QU, UK.
Susan RingPopulation Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS8 1QU, UK.
S Hasan ArshadClinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, SO17 1BJ, UK.
Susan L EwartCollege of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA.
John W HollowayHuman Development and Health, Faculty of Medicine, University of Southampton, Southampton, SO17 1BJ, UK.
University of Bristol · GBUniversity of Memphis · USUniversity of Southampton · GBCranfield University · GBEmory University · USMichigan State University · USSt Mary's Hospital · GB

Funding

Does epigenetic methylation explain the gender-switch in adolescent asthma?R01AI121226 · NIAID · UNIVERSITY OF MEMPHIS · PI HOLLOWAY, JOHN W, ZHANG, HONGMEI · 2016 to 2020
$3.2M
Transgenerational epigenetic inheritance of allergy in a multigenerational cohortR01AI091905 · NIAID · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI KARMAUS, WILFRIED JJ · 2011 to 2015
$2.7M
Biotechnology and Biological Sciences Research Council BB/I025263/1Biotechnology and Biological Sciences Research Council BBI025751/1Medical Research Council G9815508Medical Research Council MC_PC_15018Medical Research Council MC_PC_19009Medical Research Council MC_UU_00011/5Medical Research Council MC_UU_12013/1Medical Research Council MC_UU_12013/2Medical Research Council MC_UU_12013/8NIAID NIH HHS R01 AI091905NIAID NIH HHS R01 AI121226Wellcome Trust 102215/2/13/2
6 · The paper itself

Abstract

backgroundAdolescence is a period characterized by major biological development, which may be associated with changes in DNA methylation (DNA-M). However, it is unknown to what extent DNA-M varies from pre- to post-adolescence, whether the pattern of changes is different between females and males, and how adolescence-related factors are associated with changes in DNA-M.

methodsGenome-scale DNA-M at ages 10 and 18 years in whole blood of 325 subjects (n = 140 females) in the Isle of Wight (IOW) birth cohort was analyzed using Illumina Infinium arrays (450K and EPIC). Linear mixed models were used to examine DNA-M changes between pre- and post-adolescence and whether the changes were gender-specific. Adolescence-related factors and environmental exposure factors were assessed on their association with DNA-M changes. Replication of findings was attempted in the comparable Avon Longitudinal Study of Parents and Children (ALSPAC) cohort.

resultsIn the IOW cohort, after controlling for technical variation and cell compositions at both pre- and post-adolescence, 15,532 cytosine-phosphate-guanine (CpG) sites (of 400,825 CpGs, 3.88%) showed statistically significant DNA-M changes from pre-adolescence to post-adolescence invariant to gender (false discovery rate (FDR) = 0.05). Of these 15,532 CpGs, 10,212 CpGs (66%) were replicated in the ALSPAC cohort. Pathway analysis using Ingenuity Pathway Analysis (IPA) identified significant biological pathways related to growth and development of the reproductive system, emphasizing the importance of this period of transition on epigenetic state of genes. In addition, in IOW, we identified 1179 CpGs with gender-specific DNA-M changes. In the IOW cohort, body mass index (BMI) at age 10 years, age of growth spurt, nonsteroidal drugs use, and current smoking status showed statistically significant associations with DNA-M changes at 15 CpGs on 14 genes such as the AHRR gene. For BMI at age 10 years, the association was gender-specific. Findings on current smoking status were replicated in the ALSPAC cohort.

conclusionAdolescent transition is associated with changes in DNA-M at more than 15K CpGs. Identified pathways emphasize the importance of this period of transition on epigenetic state of genes relevant to cell growth and immune system development.

Indexed as

Adolescent DevelopmentDNA MethylationAdolescentBody Mass IndexChildCohort StudiesCpG IslandsEpigenesis, GeneticEpigenomicsFemaleHumansMaleSex CharacteristicsAdolescenceALSPACDNA methylationEpigeneticGenderIOWPubertyStabilityWhole-genome

Identifiers

PMID31791392
PMCPMC6888960
OpenAlexW2991333799

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.