ArticleBMC plant biology2019
Transcriptomic and microstructural analyses in Liriodendron tulipifera Linn. reveal candidate genes involved in nectary development and nectar secretion.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- Two Indel Variations in the Regulated Carotenoid Pigment Gene Hold the Key to the Interspecies Flower Colour Differentiation in Liriodendron Plants.Plant biotechnology journal · 2026Article
- Nectary Structure and Nectar Secretion Characteristics Among Various Cultivars ofPlants (Basel, Switzerland) · 2026Article
- Augmenting plant-pollinator interactions to promote biodiversity and global food security.Frontiers in plant science · 2026Review
- Genome-wide analysis of the CCT gene family in Liriodendron chinense highlights potential functions in light signaling and chlorophyll biosynthesis.BMC plant biology · 2025Article
- Nectary starch degradation affects nectar chemical composition, but not nectar sugars, in Arabidopsis thaliana.Plant physiology · 2025Article
- The developmental basis of floral nectary diversity and evolution.The New phytologist · 2025Review
- Plant AT-rich protein and zinc-binding protein (PLATZ) family in Dendrobium huoshanense: identification, evolution and expression analysis.BMC plant biology · 2024Article
- Metabolic and transcriptomic analyses of nectaries reveal differences in the mechanism of nectar production between monocots (Ananas comosus) and dicots (Nicotiana tabacum).BMC plant biology · 2024Article
- Identification, Phylogenetic and Expression Analyses of theInternational journal of molecular sciences · 2022Article
- Identification and Expression Analysis of R2R3-MYB Family Genes Associated with Salt Tolerance inInternational journal of molecular sciences · 2022Article
- Genome-Wide Identification and Expression Analysis ofInternational journal of molecular sciences · 2021Article
- Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov.BMC plant biology · 2021Article
- Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron.Scientific reports · 2021Article
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3 authors at 1 institution in 1 country.
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Abstract
backgroundNectar is a major floral attractant and reward for insects that ensures pollination. Liriodendron, a genus of the Magnoliaceae family, includes only two relict species, L. chinense and L. tulipifera, which are considered "basal angiosperms" according to plant evolutionary history. The flowers of Liriodendron plants are insect pollinated and secrete nectar to attract pollinators. To date, the morphology and anatomy of nectaries, the mechanism of nectar secretion and the molecular mechanism of nectary development in Liriodendron remain poorly understood.
methodsIn this study, we examined the nectary surface cells and change in starch in L. tulipifera by using scanning electron microscopy and periodic acid-Schiff techniques to select appropriate samples for subsequent research. Transcriptome sequencing was of the top and middle parts of immature nectaries and the middle part of mature and postsecretory nectaries in L. tulipifera was performed. We evaluated the expression profiles of 21 DEGs that are closely related to nectary development and nectar secretion for real-time quantitative PCR analysis.
resultsL. tulipifera nectaries are starch-storing nectaries and are located in the top and middle parts of L. tulipifera petals. After analyzing the RNA-seq data, we obtained 115.26 Gb of clean data in 12 libraries and mapped the results to the L. chinense reference genome with 71.02-79.77% efficiency. In total, 26,955 DEGs were identified by performing six pairwise comparisons. The flavonoid biosynthesis, phenylpropanoid biosynthesis, anthocyanin biosynthesis and starch and sucrose metabolism pathways were enriched and related to nectar secretion and pigment change. We identified 56 transcription factor families, and members of the TCP, Trihelix, C2H2, ERF, and MADS families changed dynamically during nectary development. Moreover, to further verify the accuracy of the RNA-seq results, we validated the expression profiles of 21 candidate genes.
conclusionsWe evaluated the nectary development and secretion processes comprehensively and identified many related candidate genes in L. tulipifera. These findings suggest that nectaries play important roles in flavonoid synthesis and petal color presentation.
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