Evidence map›Paper›PMID 31790614›Full record

ArticleJournal of enzyme inhibition and medicinal chemistry2020

Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH.

Mariantonia Logozzi, Davide Mizzoni, Clemente Capasso, Sonia Del Prete, Rossella Di Raimo, Mario Falchi, Daniela F Angelini, Alessandro Sciarra, Martina Maggi, Claudiu T Supuran and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of enzyme inhibition and medicinal chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

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  8. Exosomes in Cancer: Diagnostic and Therapeutic Applications.Clinical Medicine Insights. Oncology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Mariantonia LogozziDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
Davide MizzoniDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
Clemente CapassoNational Research Council, Institute of Biosciences and BioResources, Naples, Italy.ORCID http://orcid.org/0000-0003-3314-2411
Sonia Del PreteNational Research Council, Institute of Biosciences and BioResources, Naples, Italy.
Rossella Di RaimoDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
Mario FalchiNational AIDS Center, Istituto Superiore di Sanità, Rome, Italy.
Daniela F AngeliniNeuroimmunology Unit, IRCCS Santa Lucia Foundation, Rome, Italy.
Alessandro SciarraDepartment of Urology, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
Martina MaggiDepartment of Urology, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
Claudiu T SupuranNEUROFARBA Department, University of Florence, Section of Pharmaceutical Chemistry, Florence, Italy.ORCID http://orcid.org/0000-0003-4262-0323
Stefano FaisDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
Istituto Superiore di Sanità · ITInstitute of Biosciences and Bioresources · ITPoliclinico Umberto I · ITFondazione Santa Lucia · ITUniversity of Florence · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acidity, hypoxia and increased release of exosomes are severe phenotypes of tumours. The regulation of pH in tumours involves the interaction of several proteins, including the carbonic anhydrases which catalyze the formation of bicarbonate and protons from carbon dioxide and water. Among CA isoforms, CA IX is over-expressed in a large number of solid tumours, conferring to cancer cells a survival advantage in hypoxic and acidic microenvironment, but there isn't evidence that CA IX expression could have a real clinical impact. Therefore, in this study for the first time the expression and activity of CA IX have been investigated in the plasmatic exosomes obtained from patients with prostate carcinoma (PCa). For this purpose, the study was performed through different methodological approaches, such as NTA, western blot analysis, enzyme activity assay, Nanoscale flow cytometry, ELISA, confocal microscopy. The results showed that PCa exosomes significantly overexpressed CA IX levels and related activity as compared to healthy donors. Furthermore, CA IX expression and activity were correlated to the exosome intraluminal pH, demonstrating for the first time that PCa exosomes are acidic. Our data suggest the possible use of the exosomal CA IX expression and activity as a biomarker of cancer progression in PCa.

Indexed as

AgedAntigens, NeoplasmCarbonic Anhydrase IXCell LineExosomesHumansHydrogen-Ion ConcentrationMaleMicroscopy, ConfocalMiddle AgedProstatic NeoplasmsAntigens, NeoplasmCA9 protein, humanCarbonic Anhydrase IXAcidic tumour microenvironmentbiomarkercarbonic anhydrase IXplasmatic exosomesprostate cancer

Identifiers

PMID31790614
PMCPMC6896418
OpenAlexW2992878140

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.