ArticlePloS one2019
HeLa-CCL2 cell heterogeneity studied by single-cell DNA and RNA sequencing.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Advancements in single-cell sequencing for cervical cancer research.Molecular and cellular biochemistry · 2026Review
- Combined Hesperidin and Doxorubicin Treatment Induces Apoptosis and Modulates Inflammatory Cytokines in HeLa Cervical Cancer Cells.International journal of molecular sciences · 2025Article
- Single-cell and bulk transcriptome profiling reveals RNA-binding protein regulatory programs in cervical cancer.Scientific reports · 2025Article
- Article
- Cells stably expressing shRNA against MYO10 display altered cell motility.microPublication biology · 2025Article
- Article
- Modification of Seurat v4 for the Development of a Phase Assignment Tool Able to Distinguish between G2 and Mitotic Cells.International journal of molecular sciences · 2024Article
- Detection of ac4C in human mRNA is preserved upon data reassessment.Molecular cell · 2024Article
- Article
- Protein kinase C activity modulates nuclear Lamin A/C dynamics in HeLa cells.Scientific reports · 2024Article
- Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel.Molecular systems biology · 2023Article
- scGAD: single-cell gene associating domain scores for exploratory analysis of scHi-C data.Bioinformatics (Oxford, England) · 2022Article
- shRNAs targeting mouse Adam10 diminish cell response to proinflammatory stimuli independently of Adam10 silencing.Biology open · 2022Article
- Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation.Molecular systems biology · 2022Article
- Data-driven identification of inherent features of eukaryotic stress-responsive genes.NAR genomics and bioinformatics · 2022Article
- Single-cell Transcriptomes Reveal Characteristics of MicroRNAs in Gene Expression Noise Reduction.Genomics, proteomics & bioinformatics · 2021Article
- Multicellular 3D Models to Study Tumour-Stroma Interactions.International journal of molecular sciences · 2021Review
- Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells.Stem cell research & therapy · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The HeLa cells are the earliest and mostly used laboratory human cells for biomedical particularly cancer research. They were derived from a patient's cervical cancerous tissue, and are known for their heterogeneous cellular origin and variable genomic landscapes. Single-cell sequencing techniques with faithful linear and uniformly amplified genomes (DNA) and transcriptomes (RNA) may facilitate the study of cellular differences at the individual cell level. In this work, we have performed single-cell DNA and RNA sequencing with HeLa-CCL2 cells to study their heterogeneity. We have studied the complexity of copy number variations (CNVs) of HeLa-CCL2 genome at the single cell level, and revealed the transcriptomic heterogeneity of HeLa-CCL2. We also analyzed the relationship between genome and transcriptome at the single-cell level, and found overall correlation between CNV and transcriptome expression patterns. Finally, we concluded that although single-cell sequencing techniques are applicable to study heterogeneous cells such as HeLa-CCL2, the data analyses need to be more careful and well controlled.
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