ArticleBiotechnology and bioengineering2020
A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae.
Article in Biotechnology and bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use inACS synthetic biology · 2026Article
- Characterizing Optogenetic Tools for Use in Synthetic Gene Circuits.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Probiotic-Based Materials as Living Therapeutics.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.BMC biotechnology · 2025Article
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- The Yeast Optogenetic Toolkit (yOTK) for Spatiotemporal Control of Gene Expression in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Dynamic Multiplexed Control and Modeling of Optogenetic Systems Using the High-Throughput Optogenetic Platform, Lustro.ACS synthetic biology · 2024Article
- POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology.ACS synthetic biology · 2024Article
- A Standardized Set of MoClo-Compatible Inducible Promoter Systems for Tunable Gene Expression in Yeast.ACS synthetic biology · 2024Article
- 'Seeing' the electromagnetic spectrum: spotlight on the cryptochrome photocycle.Frontiers in plant science · 2024Review
- Controlling the Potency of T Cell Activation Using an Optically Tunable Chimeric Antigen Receptor.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advances in the dynamic control of metabolic pathways inEngineering microbiology · 2023Review
- A Multiplex MoClo Toolkit for Extensive and Flexible Engineering ofACS synthetic biology · 2023Article
- Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit.ACS synthetic biology · 2023Article
- PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes inACS synthetic biology · 2023Article
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Standardization of Synthetic Biology Tools and Assembly Methods forACS synthetic biology · 2022Review
- Optogenetic control of NOTCH1 signaling.Cell communication and signaling : CCS · 2022Article
- Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae.Communications biology · 2022Article
- Expanding the molecular versatility of an optogenetic switch in yeast.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Optogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with light. Here we develop an optogenetic system for gene expression control integrated with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae. We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization mediated by the proteins CRY2 and CIB1. We optimize function of this split TF and demonstrate the utility of the toolkit workflow by assembling cassettes expressing the TF activation domain and DNA-binding domain at different levels. Utilizing this TF and a synthetic promoter we demonstrate that light intensity and duty cycle can be used to modulate gene expression over the range currently available from natural yeast promoters. This study allows for rapid generation and prototyping of optogenetic circuits to control gene expression in S. cerevisiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.