ArticleProbiotics and antimicrobial proteins2020
Comparison of Different Nisin Separation and Concentration Methods: Industrial and Cost-Effective Perspectives.
Article in Probiotics and antimicrobial proteins, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 15 citations in OpenAlex.
- Valorisation of acid whey permeate for high-purity nisin Z production using artisanal Lactococcus lactis isolates.BMC microbiology · 2025Article
- Food Waste Biotransformation into Food Ingredients: A Brief Overview of Challenges and Opportunities.Foods (Basel, Switzerland) · 2024Review
- Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications.Heliyon · 2024Review
- Nisin Purification from a Cell-Free Supernatant by Electrodialysis in a Circular Economy Framework.Membranes · 2023Article
- Purification, characterization, and mode of action of Sakacin ZFM225, a novel bacteriocin fromBiochemistry and biophysics reports · 2023Article
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3 authors at 2 institutions in 1 country.
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Abstract
Separation and concentration of biotechnological products are the most important steps of purification stage in downstream processing. In the present study, three nisin separation and concentration methods including salting out by ammonium sulfate, solvent extraction (at 20 °C, 4 °C, and - 10 °C) and direct chromatography of culture medium were compared with each other. According to our results, nisin precipitation by ammonium sulfate at 40% saturation was the most efficient method (yield = 90.04%, purification fold = 168.80). Low yield and fold purification values were obtained by solvent extraction with chloroform (yield = 24.23%, fold purification = 37.43 at - 10 °C) and direct cation exchange chromatography of culture medium (yield = 20.00%, fold purification = 1.80). Also, performing purification steps in low pH values and acidic conditions (pH = 3.0) is essential for efficient nisin purification.
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