Evidence map›Paper›PMID 31788134›Full record

ArticleChemCatChem2019

Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity.

Elizabeth A Caselle, Jennifer H Yoon, Sagar Bhattacharya, Joel J L Rempillo, Zsófia Lengyel, Areetha D'Souza, Yurii S Moroz, Patricia L Tolbert, Alexander N Volkov, Marcello Forconi and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemCatChem, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Structure-activity relationships in catalytic amyloids.Journal of inorganic biochemistry · 2026
    Article
  2. Article
  3. Article
  4. MinimalistACS catalysis · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Elizabeth A CaselleDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Jennifer H YoonDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Sagar BhattacharyaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Joel J L RempilloDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Zsófia LengyelDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Areetha D'SouzaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Yurii S MorozDepartment of Chemistry, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., Kyiv 01601, Ukraine.
Patricia L TolbertDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Alexander N VolkovVIB Centre for Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Pleinlaan 2, Brussels 1050, Belgium.
Marcello ForconiDepartment of Chemistry and Biochemistry, College of Charleston, 66 George St. Charleston, SC 29424, USA.
Carlos A CastañedaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Olga V MakhlynetsDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
Ivan V KorendovychDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.

Funding

South Carolina IDeA Networks of Biomedical Research (SC INBRE V)P20GM103499 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI EDIE C GOLDSMITH · 2012 to 2026
$61.0M
Supplement for South Carolina BRIN - 1P20 RR16461P20RR016461 · NCRR · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI DEAVOR, JAMES · 2001 to 2011
$32.3M
Understanding Evolution of Protein Function Through Design.R35GM119634 · NIGMS · SYRACUSE UNIVERSITY · PI KORENDOVYCH, IVAN V · 2016 to 2025
$4.3M
A 800 MHz Nuclear Magnetic Resonance Spectrometer in Support of Life Science ReseS10OD012254 · OD · UPSTATE MEDICAL UNIVERSITY · PI WILKENS, STEPHAN · 2013 to 2013
$2.0M
NCRR NIH HHS P20 RR016461NIGMS NIH HHS P20 GM103499NIGMS NIH HHS R35 GM119634NIH HHS S10 OD012254
6 · The paper itself

Abstract

Minimalist enzymes designed to catalyze model reactions provide useful starting points for creating catalysts for practically important chemical transformations. We have shown that Kemp eliminases of the AlleyCat family facilitate conversion of leflunomide (an immunosupressor pro-drug) to its active form teriflunomide with outstanding rate enhancement (nearly four orders of magnitude) and catalytic proficiency (more than seven orders of magnitude) without any additional optimization. This remarkable activity is achieved by properly positioning the substrate in close proximity to the catalytic glutamate with very high pK

Indexed as

allosterycalmodulinKemp eliminationNuclear Magnetic Resonanceprotein design

Identifiers

PMID31788134
PMCPMC6884320

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.