Evidence map›Paper›PMID 31788019›Full record

ArticlePlant methods2019

High-throughput long paired-end sequencing of a Fosmid library by PacBio.

Zhaozhao Dai, Tong Li, Jiadong Li, Zhifei Han, Yonglong Pan, Sha Tang, Xianmin Diao, Meizhong Luo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhaozhao Dai1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Tong Li1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Jiadong Li1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Zhifei Han1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Yonglong Pan1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.
Sha Tang2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 10081 China.
Xianmin Diao2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 10081 China.
Meizhong Luo1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLarge insert paired-end sequencing technologies are important tools for assembling genomes, delineating associated breakpoints and detecting structural rearrangements. To facilitate the comprehensive detection of inter- and intra-chromosomal structural rearrangements or variants (SVs) and complex genome assembly with long repeats and segmental duplications, we developed a new method based on single-molecule real-time synthesis sequencing technology for generating long paired-end sequences of large insert DNA libraries.

resultsA Fosmid vector, pHZAUFOS3, was developed with the following new features: (1) two 18-bp non-palindromic I

conclusionsWe developed a new method for long paired-end sequencing of large insert libraries, which can efficiently improve the quality of de novo genome assembly and identify large and small structural rearrangements or assembly errors.

Indexed as

Ampicillin resistance gene tagAssembly errorDe novo assemblyFosmidLong paired-endMate-pairPacBioStructural rearrangement

Identifiers

PMID31788019
PMCPMC6878638

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.