Evidence map›Paper›PMID 31786792›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Paired Recording to Study Electrical Coupling Between Photoreceptors in Mouse Retina.

Nange Jin, Zhijing Zhang, Kimberly A Mankiewicz, Christophe P Ribelayga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nange JinRuiz Department of Ophthalmology and Visual Science, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA.
Zhijing ZhangRuiz Department of Ophthalmology and Visual Science, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA.
Kimberly A MankiewiczRuiz Department of Ophthalmology and Visual Science, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA.
Christophe P RibelaygaRuiz Department of Ophthalmology and Visual Science, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA. Christophe.P.Ribelayga@Uth.Tmc.Edu.

Funding

UTHouston Core Center for Vision ResearchP30EY028102 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI MASSEY, STEPHEN C · 2017 to 2021
$3.1M
Rod/cone gap junctions initiate an irradiance pathwayR01EY029408 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI MASSEY, STEPHEN C, RIBELAYGA, CHRISTOPHE P. · 2018 to 2022
$2.9M
Circadian Clock Function in the Mammalian RetinaR01EY018640 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI RIBELAYGA, CHRISTOPHE P. · 2009 to 2015
$2.0M
The role of circadian clocks in photoreceptor cell development, maintenance and functionR21EY028647 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI RIBELAYGA, CHRISTOPHE P., WU-HUBER, JIAQIAN · 2018 to 2019
$424k
NEI NIH HHS P30 EY028102NEI NIH HHS R01 EY018640NEI NIH HHS R01 EY029408NEI NIH HHS R21 EY028647
6 · The paper itself

Abstract

Gap junction-mediated electrical coupling between retinal photoreceptors is an important determinant of photoreceptor function. Yet, quantitative measurements of the junctional conductance between coupled photoreceptors are required to fully assess the effects of coupling on visual performance. Such measurements have been obtained in salamander and other lower vertebrate retinas but are difficult to acquire in mammalian retinas, in part because of the much smaller size of photoreceptors in mammals. Here, we describe in detail a dual whole-cell patch-clamp technique we recently developed to measure the junctional conductance between photoreceptor pairs in the mouse retina. With this method, electrical coupling strength between mouse photoreceptors can be estimated with high accuracy and its impact on retinal processing of visual information further evaluated.

Indexed as

AnimalsCaudataGap JunctionsMicePatch-Clamp TechniquesPhotoreceptor Cells, VertebrateRetinaRetinal Rod Photoreceptor CellsCx36Dual patch clampElectrical couplingGap junctionMousePhotoreceptor

Identifiers

PMID31786792
PMCPMC8204347

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.