ArticleJournal of industrial microbiology & biotechnology2020
High-level expression of highly active and thermostable trehalase from Myceliophthora thermophila in Aspergillus niger by using the CRISPR/Cas9 tool and its application in ethanol fermentation.
Article in Journal of industrial microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Effect of Different Signal Peptides on the Expression of Glucoamylase fromJournal of fungi (Basel, Switzerland) · 2026Article
- CRISPR-Cas9 Gene Editing inBiology · 2025Review
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.Bioprocess and biosystems engineering · 2025Article
- Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization.BMC biotechnology · 2024Article
- Unlocking Fungal Potential: The CRISPR-Cas System as a Strategy for Secondary Metabolite Discovery.Journal of fungi (Basel, Switzerland) · 2024Review
- Expression of microbial lipase in filamentous fungus3 Biotech · 2024Review
- Enhancement of protein production in Aspergillus niger by engineering the antioxidant defense metabolism.Biotechnology for biofuels and bioproducts · 2024Article
- Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila.Microbial cell factories · 2023Article
- Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an EngineeredBioengineering (Basel, Switzerland) · 2023Article
- Heterologous protein production in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger.Journal of industrial microbiology & biotechnology · 2022Article
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
- Article
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Characterization and significance of extracellular polymeric substances, reactive oxygen species, and extracellular electron transfer in methanogenic biocathode.Scientific reports · 2021Article
- Heterologous Expression of Lignocellulose-Modifying Enzymes in Microorganisms: Current Status.Molecular biotechnology · 2021Review
- Advances in Genetic Engineering Technology and Its Application in the Industrial FungusFrontiers in microbiology · 2021Review
- Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Trehalase catalyzes the hydrolysis of the non-reducing disaccharide trehalose. The highly active trehalase MthT from Myceliophthora thermophila was screened from the trehalase genes of six species of filamentous fungi. An ingenious multi-copy knock-in expression strategy mediated by the CRISPR/Cas9 tool and medium optimization were used to improve MthT production in Aspergillus niger, up to 1698.83 U/mL. The protein background was dramatically abated due to insertion. The recombinant MthT showed optimal activity at pH 5.5 and 60 °C, and exhibited prominent thermal stability between 50 and 60 °C under acid conditions (pH 4.5-6.5). The ethanol conversion rate (ethanol yield/total glucose) was significantly improved by addition of MthT (51.88%) compared with MthT absence (34.38%), using 30% starch saccharification liquid. The results of this study provided an effective strategy, established a convenient platform for heterologous expression in A. niger and showed a potential strategy to decrease production costs in industrial ethanol production.
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