Evidence map›Paper›PMID 31785817›Full record

ArticleBiochemical and biophysical research communications2020

Two microPeptides are translated from a KSHV polycistronic RNA in human cells by leaky scanning mechanism.

Lei Xu, Yan Yuan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lei XuInstitute of Human Virology and Ministry of Education Key Laboratory of Tropical Disease Control, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Yan YuanInstitute of Human Virology and Ministry of Education Key Laboratory of Tropical Disease Control, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China; Department of Microbiology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, 19104, USA. Electronic address: yuan2@upenn.edu.

Funding

KSHV-encoded Small PeptitesR21AI104473 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI YUAN, YAN · 2013 to 2014
$432k
NIAID NIH HHS R21 AI104473
6 · The paper itself

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 3.0 kb polyadenylated RNA (T3.0) in the opposite strand of the open reading frame 50 (RTA) gene. The T3.0 was mis-annotated as a noncoding RNA but found to be associated with ribosomes and carries at least four translatable sORFs. Two of them, namely vSP-1 and vSP-2, have been characterized. vSP-1 enhances RTA expression by blocking RTA self-ubiquitylation and proteasome-associated degradation. T3.0 RNA is a polycistronic RNA. Furthermore, polycistronic translation has been observed in most of the cases of small peptides (microPeptides) translated from previously annotated noncoding RNAs in eukaryotes. In an effort to elucidate the mechanism underlying polycistronic sORF translation in eukaryotic cells, we found that T3.0 RNA translates vSP-1 and vSP-2 through a leaky scanning mechanism.

Indexed as

Gene Expression Regulation, ViralHEK293 CellsHerpesviridae InfectionsHerpesvirus 8, HumanHumansOpen Reading FramesPeptidesProtein BiosynthesisRNA, MessengerRNA, ViralViral ProteinsPeptidesRNA, MessengerRNA, ViralViral ProteinsKaposi’s sarcoma-associated herpesvirus (KSHV)Leaky scanningMicroPeptidePolycistronic translationT3.0

Identifiers

PMID31785817
PMCPMC6981015

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.