Evidence map›Paper›PMID 31785564›Full record

ArticleWater research2020

Use of qPCR and RT-qPCR for monitoring variations of microcystin producers and as an early warning system to predict toxin production in an Ohio inland lake.

Jingrang Lu, Ian Struewing, Larry Wymer, Daniel R Tettenhorst, Jody Shoemaker, Joel Allen

Open access · greenAbstract read
In one paragraph

Article in Water research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 71 citations in OpenAlex.

  1. Review
  2. Article
  3. Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Article
  4. Article
  5. Intraspecific divergence withinProceedings. Biological sciences · 2025
    Article
  6. Nicorandil reduces the antimicrobial effectiveness of polymyxin E againstFrontiers in cellular and infection microbiology · 2025
    Article
  7. Early Detection Methods for Toxic Cyanobacteria Blooms.Pathogens (Basel, Switzerland) · 2024
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Jingrang LuOffice of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, 45268, USA. Electronic address: lu.jingrang@epa.gov.
Ian StruewingPegasus Technical Services Inc, Cincinnati, OH, 45268, USA.
Larry WymerOffice of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, 45268, USA.
Daniel R TettenhorstOffice of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, 45268, USA.
Jody ShoemakerOffice of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, 45268, USA.
Joel AllenOffice of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, 45268, USA.
Environmental Protection Agency · USApplied Technical Services (United States) · US

Funding

Intramural EPA EPA999999
6 · The paper itself

Abstract

Public concern over cyanobacterial blooms has increased due to their higher frequency of occurrence and their potential ecological and health impacts. Detection of microcystin (MC) producers (MCPs) using qPCR and RT-qPCR allows for the rapid identification of blooms by combining specificity and sensitivity with a relatively high throughput capability. Investigation of MCP population composition (correlation, dominance), toxin gene expression, and relationship to MC concentration was conducted using a panel of qPCR assays targeting mcyA, E and G on weekly and daily water samples collected from an Ohio inland reservoir lake. Further, these data were used to develop early warning thresholds for prediction of MC concentrations exceeding the US EPA Health Advisory cutoff value (>0.3 μg L

Indexed as

MicrocystinsMicrocystisChromatography, LiquidLakesOhioTandem Mass SpectrometryMicrocystinsHarmful algal bloomMicrocystinMolecular detectionPrewarningToxic cyanobacteria

Identifiers

PMID31785564
PMCPMC7075668
OpenAlexW2982769228

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.