ArticleThe FEBS journal2020
On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5).
Article in The FEBS journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Understanding molecular mechanisms driving cadmium-induced mitochondrial dysfunction in human metabolic liver disease.Environmental toxicology and pharmacology · 2026Review
- Extracellular Redox Balance as a Determinant of Immune Regulation and Tissue Inflammation.Antioxidants (Basel, Switzerland) · 2026Review
- Unraveling the interplay between RAS axes and NOX signaling in fibroblasts during cardiac fibrosis.Molecular and cellular biochemistry · 2026Review
- Harnessing hollow Prussian blue nanozymes for efficient photothermal lithotripsy while protecting the kidneys from oxidative stress injury.Materials today. Bio · 2025Article
- Enhancing Selectivity and Potency of SJournal of medicinal chemistry · 2025Article
- Structural basis of human NOX5 activation.Nature communications · 2024Article
- A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells.Scientific reports · 2023Article
- NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors.Journal of medicinal chemistry · 2023Review
- Multiplexed Host-Induced Gene Silencing ofToxins · 2023Article
- Distribution and diversity of ROS-generating enzymes across the animal kingdom, with a focus on sponges (Porifera).BMC biology · 2022Article
- ROS Signaling Mediates Directional Cell Elongation and Somatic Cell Fusion in the Red AlgaCells · 2022Article
- Exonic splicing code and protein binding sites for calcium.Nucleic acids research · 2022Article
- Calmodulin binding to the dehydrogenase domain of NADPH oxidase 5 alters its oligomeric state.Biochemistry and biophysics reports · 2022Article
- Microbial interaction mediated programmed cell death in plants.3 Biotech · 2022Review
- System-wide identification of myeloid markers of TB disease and HIV-induced reactivation in the macaque model of Mtb infection and Mtb/SIV co-infection.Frontiers in immunology · 2022Article
- Activity-regulated growth of motoneurons at the neuromuscular junction is mediated by NADPH oxidases.Frontiers in cellular neuroscience · 2022Article
- Physiologically Relevant Free CaJournal of molecular biology · 2021Article
- Expression of Endothelial NOX5 Alters the Integrity of the Blood-Brain Barrier and Causes Loss of Memory in Aging Mice.Antioxidants (Basel, Switzerland) · 2021Article
- Oxidative Stress and Hypertension.Circulation research · 2021Review
- NADPH Oxidase 5 Induces Changes in the Unfolded Protein Response in Human Aortic Endothelial Cells and in Endothelial-SpecificAntioxidants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
It is now accepted that reactive oxygen species (ROS) are not only dangerous oxidative agents but also chemical mediators of the redox cell signaling and innate immune response. A central role in ROS-controlled production is played by the NADPH oxidases (NOXs), a group of seven membrane-bound enzymes (NOX1-5 and DUOX1-2) whose unique function is to produce ROS. Here, we describe the regulation of NOX5, a widespread family member present in cyanobacteria, protists, plants, fungi, and the animal kingdom. We show that the calmodulin-like regulatory EF-domain of NOX5 is partially unfolded and detached from the rest of the protein in the absence of calcium. In the presence of calcium, the C-terminal lobe of the EF-domain acquires an ordered and more compact structure that enables its binding to the enzyme dehydrogenase (DH) domain. Our spectroscopic and mutagenesis studies further identified a set of conserved aspartate residues in the DH domain that are essential for NOX5 activation. Altogether, our work shows that calcium induces an unfolded-to-folded transition of the EF-domain that promotes direct interaction with a conserved regulatory region, resulting in NOX5 activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.