ArticleScientific reports2019
An exploration of ambigrammatic sequences in narnaviruses.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Mycoviruses diversity in the black kōji mold, Aspergillus luchuensis (section Nigri) isolated from liquor-production environments in Japan.Virus research · 2026Article
- Longitudinal sampling of New Zealand cockles (Austrovenus stutchburyi) reveals high viral diversity and taxa-specific responses to environmental factors.FEMS microbiology ecology · 2026Article
- A metagenomic analysis of urban river samples reveals high numbers of sequences related to mycoviruses.Archives of virology · 2026Article
- Extreme GC3 codon bias in a novel brown seaweed virus results in pseudoambigrammatic characteristics.Virus evolution · 2026Article
- A novel narnavirus isolated from the edible fungus Pholiota nameko.Archives of virology · 2025Article
- Prevalence and Diversity of Haemosporidian-Associated Matryoshka RNA Viruses in a Natural Population of Wild Birds.Ecology and evolution · 2025Article
- Viromes of Tabanids from Russia.Viruses · 2023Article
- A novel ambigrammatic mycovirus, PsV5, works hand in glove with wheat stripe rust fungus to facilitate infection.Plant communications · 2023Article
- Article
- The virome of the invasive Asian bush mosquitoVirus evolution · 2023Article
- Article
- Article
- Resolving deep evolutionary relationships within the RNA virus phylumVirus evolution · 2022Article
- High Diversity of Novel Viruses in the Tree PathogenFrontiers in microbiology · 2022Article
- Revealing RNA virus diversity and evolution in unicellular algae transcriptomes.Virus evolution · 2021Article
- Persistence of Ambigrammatic Narnaviruses Requires Translation of the Reverse Open Reading Frame.Journal of virology · 2021Article
- Article
- Polymorphism of genetic ambigrams.Virus evolution · 2021Article
- Characterization of a Novel Mitovirus of the Sand FlyViruses · 2020Article
- Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses.Viruses · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Narnaviruses have been described as positive-sense RNA viruses with a remarkably simple genome of ~3 kb, encoding only a highly conserved RNA-dependent RNA polymerase (RdRp). Many narnaviruses, however, are 'ambigrammatic' and harbour an additional uninterrupted open reading frame (ORF) covering almost the entire length of the reverse complement strand. No function has been described for this ORF, yet the absence of stops is conserved across diverse narnaviruses, and in every case the codons in the reverse ORF and the RdRp are aligned. The >3 kb ORF overlap on opposite strands, unprecedented among RNA viruses, motivates an exploration of the constraints imposed or alleviated by the codon alignment. Here, we show that only when the codon frames are aligned can all stop codons be eliminated from the reverse strand by synonymous single-nucleotide substitutions in the RdRp gene, suggesting a mechanism for de novo gene creation within a strongly conserved amino-acid sequence. It will be fascinating to explore what implications this coding strategy has for other aspects of narnavirus biology. Beyond narnaviruses, our rapidly expanding catalogue of viral diversity may yet reveal additional examples of this broadly-extensible principle for ambigrammatic-sequence development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.