ArticleScientific reports2019
Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain-derived matrix.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Methods for a bioengineered 3D human brain-like tissue model of neuroregeneration after traumatic brain injury.Neural regeneration research · 2026Article
- Focused ultrasound-induced cavitation in a human brain microphysiological system produces injury signaling biomarkers consistent with blast trauma.npj biomedical innovations · 2026Article
- Molecular abnormalities scale across three models of cerebral injury.Frontiers in molecular neuroscience · 2026Article
- Genetic Manipulation in Organoid Models and Their Applications in Central Nervous System Pathologies.Molecular neurobiology · 2025Review
- Can Molecular Attributes of Mammalian Granulosa Cells and Ovarian Putative Stem Cells Predestine Them to Be a Promising Tool for Tissue Engineering and Regenerative Medicine?International journal of molecular sciences · 2025Review
- Modelling human brain development and disease with organoids.Nature reviews. Molecular cell biology · 2025Review
- Real-Time, Non-Invasive Monitoring of Neuronal Differentiation Using Intein-Enabled Fluorescence Signal Translocation in Genetically Encoded Stem Cell-Based Biosensors.Advanced functional materials · 2024Article
- Multiscale engineering of brain organoids for disease modeling.Advanced drug delivery reviews · 2024Review
- Review
- Neuropathogenesis-on-chips for neurodegenerative diseases.Nature communications · 2024Review
- Human neuronal maturation comes of age: cellular mechanisms and species differences.Nature reviews. Neuroscience · 2024Review
- Proteomic analysis reveals key differences in pro-stromal corneal tissue between highly myopic males and females.Frontiers in medicine · 2024Article
- Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model.Cell death & disease · 2023Article
- Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells.Pharmaceutics · 2023Article
- Development of Brain-Derived Bioscaffolds for Neural Progenitor Cell Culture.ACS pharmacology & translational science · 2023Article
- Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons.Cell stem cell · 2023Article
- Advances in currentFrontiers in bioengineering and biotechnology · 2023Review
- Asteroid impact: the potential of astrocytes to modulate human neural networks within organoids.Frontiers in neuroscience · 2023Review
- Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.Development (Cambridge, England) · 2022Article
- Tissue-Engineered Models of the Human Brain: State-of-the-Art Analysis and Challenges.Journal of functional biomaterials · 2022Review
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Abstract
Brain extracellular matrix (ECM) is often overlooked in vitro brain tissue models, despite its instructive roles during development. Using developmental stage-sourced brain ECM in reproducible 3D bioengineered culture systems, we demonstrate enhanced functional differentiation of human induced neural stem cells (hiNSCs) into healthy neurons and astrocytes. Particularly, fetal brain tissue-derived ECM supported long-term maintenance of differentiated neurons, demonstrated by morphology, gene expression and secretome profiling. Astrocytes were evident within the second month of differentiation, and reactive astrogliosis was inhibited in brain ECM-enriched cultures when compared to unsupplemented cultures. Functional maturation of the differentiated hiNSCs within fetal ECM-enriched cultures was confirmed by calcium signaling and spectral/cluster analysis. Additionally, the study identified native biochemical cues in decellularized ECM with notable comparisons between fetal and adult brain-derived ECMs. The development of novel brain-specific biomaterials for generating mature in vitro brain models provides an important path forward for interrogation of neuron-glia interactions.
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