ArticleBMC microbiology2019
Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: speed and accuracy of Alfred 60AST.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Plasmonic metasurface microfluidic accelerates redox reactions for rapid phenotypic antibiotic susceptibility testing.Science advances · 2026Article
- Isolating viable bacteria from positive blood cultures: impedance cytometry analysis.Biomedical microdevices · 2026Article
- Performance of the Qvella FAST system with downstream application of MALDI-TOF-MS, VITEK 2, and β-LACTA test for same-day identification and antimicrobial susceptibility testing of Gram-negative rods directly from positive blood cultures.Microbiology spectrum · 2026Article
- Comparison of the VITEK REVEAL AST and Accelerate Pheno systems for fast antimicrobial susceptibility testing of gram-negative blood cultures at a large academic health system.Journal of clinical microbiology · 2026Article
- Clinical impact of rapid antimicrobial susceptibility testing in patients with bacteremia due to gram-negative bacilli.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2026Article
- Performance comparison and clinical validation of two rapid direct identification and antimicrobial susceptibility testing approaches for pathogens isolated from positive blood cultures in children.Frontiers in cellular and infection microbiology · 2026Article
- Diagnostic Innovations to Combat Antibiotic Resistance in Critical Care: Tools for Targeted Therapy and Stewardship.Diagnostics (Basel, Switzerland) · 2025Review
- Improving Turnaround Times for Routine Antimicrobial Sensitivity Testing Following European Committee on Antimicrobial Susceptibility Testing Methodology in Patients with Bacteraemia.Antibiotics (Basel, Switzerland) · 2024Article
- An investigation of scattered light integrating collector technology for rapid blood culture sensitivity testing.Journal of medical microbiology · 2024Article
- Evaluating the impact of rapid antimicrobial susceptibility testing for bloodstream infections: a review of actionability, antibiotic use and patient outcome metrics.The Journal of antimicrobial chemotherapy · 2024Review
- Getting Up to Speed: Rapid Pathogen and Antimicrobial Resistance Diagnostics in Sepsis.Microorganisms · 2024Review
- Rapid Phenotypic and Genotypic Antimicrobial Susceptibility Testing Approaches for Use in the Clinical Laboratory.Antibiotics (Basel, Switzerland) · 2024Review
- Evaluation of Direct Antimicrobial Susceptibility Testing of Gram-Negative Bacilli andMicroorganisms · 2024Article
- Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform.Nature communications · 2024Article
- Rapid Drug Susceptibility Testing to Preserve Antibiotics.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Ultrasensitive and rapid identification of ESRI developer- and piperacillin/tazobactam-resistantEmerging microbes & infections · 2022Article
- Semirapid Detection of Piperacillin/Tazobactam Resistance and Extended-Spectrum Resistance to β-Lactams/β-Lactamase Inhibitors in Clinical Isolates of Escherichia coli.Microbiology spectrum · 2021Article
- Performance evaluation of AlfredEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Rapid Methods for Antimicrobial Resistance Diagnostics.Antibiotics (Basel, Switzerland) · 2021Review
- Rapid Antimicrobial Susceptibility Testing Methods for Blood Cultures and Their Clinical Impact.Frontiers in medicine · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBlood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16 to 25 h to 5-6 h, transforming patient care. To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared .
resultsA total of 2196 antimicrobial susceptibility test results (AST) were performed: 1863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and 89% (295/333) for Gram-positive isolates. Gram-negative CA: ampicillin 96% (290/301); ciprofloxacin 95% (283/297); ceftriaxone 96% (75/78); meropenem 97% (288/297); piperacillin-tazobactam 95% (280/295); gentamicin 94% (279/297) and amikacin 93% (277/298). The median time to susceptibility results from blood culture flagging positive was 6.3 h vs 20 h (p < 0.01) for Alfred system vs BD Phoenix™.
conclusionAlfred 60 AST system greatly reduced time to antimicrobial susceptibility results in Gram-negative and Gram-positive BSIs with good performance and cost, particularly for Gram-negative bacteraemia.
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Registered trials
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