ArticleBMC microbiology2019
A new culture-based method for rapid identification of microorganisms in polymicrobial blood cultures by MALDI-TOF MS.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Clinical application of MALDI-TOF MS for direct and rapid pathogen identification: a comprehensive review focusing on bloodstream infections and sterile body fluids.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Evaluation of three rapid antimicrobial susceptibility testing methods directly from positive blood cultures integrated with rapid pathogen identification.BMC microbiology · 2026Article
- MALDI-TOF MS, following separation gel coagulation tubes and short-term solid phase culture effectively detects pathogenic microbes from positive blood cultures.Open medicine (Warsaw, Poland) · 2026Article
- Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.Biomicrofluidics · 2025Review
- Application of matrix-assisted laser desorption/ionization time-of flight mass spectrometry in clinical testing and diagnosis.Frontiers in cellular and infection microbiology · 2025Review
- Multiplex microarray PCR Unyvero BCU system to accelerate relevant antimicrobial treatment in polymicrobial bloodstream infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Short turnaround time of seven to nine hours from sample collection until informed decision for sepsis treatment using nanopore sequencing.Scientific reports · 2024Article
- Non-electrostatic interactions associated with aggregate formation between polyallylamine and Escherichia coli.Scientific reports · 2023Article
- Methods for detection and identification of beer-spoilage microbes.Frontiers in microbiology · 2023Review
- The Development of Technology to Prevent, Diagnose, and Manage Antimicrobial Resistance in Healthcare-Associated Infections.Vaccines · 2022Review
- A novel rRNA hybridization-based approach to rapid, accurate Candida identification directly from blood culture.Medical mycology · 2022Article
- Differentiating betweenFoods (Basel, Switzerland) · 2022Article
- Diversity of a Lactic Acid Bacterial Community during Fermentation of Gajami-Sikhae, a Traditional Korean Fermented Fish, as Determined by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Foods (Basel, Switzerland) · 2022Article
- Application and Perspectives of MALDI-TOF Mass Spectrometry in Clinical Microbiology Laboratories.Microorganisms · 2021Review
- Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification.Clinics in laboratory medicine · 2021Review
- Properties ofGlobal pediatric health · 2021Article
- Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy.Frontiers in microbiology · 2021Review
- Emergence ofVeterinary world · 2020Article
- Direct Blood Culturing ofJournal of clinical microbiology · 2020Article
- Mass spectrometry-based microbiological testing for blood stream infection.Clinical proteomics · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to microbial identification has allowed the development of rapid methods for identification of microorganisms directly in positive, blood cultures (BCs). These methods can yield accurate results for monomicrobial BCs, but often fail to identify multiple microorganisms in polymicrobial BCs. The present study was aimed at establishing a rapid and simple method for identification of bacteria and yeast in polymicrobial BCs from patients with bloodstream infection.
resultsThe rapid method herein proposed is based on short-term culture in liquid media allowing selective growth of microorganisms recovered from polymicrobial BCs, followed by rapid identification by MALDI-TOF MS. To evaluate the accuracy of this method, 56 polymicrobial BCs were comparatively analyzed with the rapid and routine methods. The results showed concordant identification for both microbial species in 43/50 (86%) BCs containing two different microorganisms, and for two microbial species in six BCs containing more than two different species. Overall, 102/119 (85.7%) microorganisms were concordantly identified by the rapid and routine methods using a cut-off value of 1.700 for valid identification. The mean time to identification after BC positivity was about 4.2 h for streptococci/enterococci, 8.7 h for staphylococci, 11.1 h for Gram-negative bacteria, and 14.4 h for yeast, allowing a significant time saving compared to the routine method.
conclusionsThe proposed method allowed rapid and reliable microbial identification in polymicrobial BCs, and could provide clinicians with timely, useful information to streamline empirical antimicrobial therapy in critically ill patients.
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