Evidence map›Paper›PMID 31783771›Full record

ArticleParasites & vectors2019

Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase.

Jing-Tong Zheng, Nan Zhang, Yan-Hui Yu, Peng-Tao Gong, Xian-He Li, Na Wu, Can Wang, Xiao-Cen Wang, Xin Li, Jian-Hua Li and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Frontiers in cellular and infection microbiology · 2025
    Review
  2. Giardia telomeres and telomerase.Parasitology research · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jing-Tong ZhengKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Nan ZhangKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Yan-Hui YuClinical Laboratory of Second Hospital, Jilin University, Changchun, 130021, China.
Peng-Tao GongKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Xian-He LiKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Na WuKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Can WangKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Xiao-Cen WangKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Xin LiKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Jian-Hua LiKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China. jianhuali7207@163.com.
Xi-Chen ZhangKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Institute of Zoonosis, Jilin University, Changchun, 130062, China. xczhang@jlu.edu.cn.
Jilin University · CNSecond Affiliated Hospital of Jilin University · CN

Funding

Department of Science and Technology of Jilin Province 20190103075JHNational Natural Science Foundation of China 31272550National Natural Science Foundation of China 31672288National Natural Science Foundation of China 31772732
6 · The paper itself

Abstract

backgroundGiardia duodenalis causes giardiasis, with diarrhea as the primary symptom. The trophozoite proliferation of this zoonotic parasite is mainly affected by telomerase, although the mechanism of telomerase regulation has not been thoroughly analyzed.

methodsThis study was performed to identify the telomerase RNA-binding domain (TRBD)-interacting protein in G. duodenalis and its regulation of telomerase. Interaction between TRBD and interacting proteins was verified via pulldown assays and co-immunoprecipitation (co-IP) techniques, and the subcellular localization of the protein interactions was determined in vivo via split SNAP-tag labeling. The hammerhead ribozyme was designed to deplete the mRNA of TRBD-interacting proteins.

resultsUsing TRBD as bait, we identified zinc-finger domain (ZFD)-containing proteins and verified it via pulldown and co-IP experiments. Protein-protein interaction occurred in the nuclei of 293T cells and both nuclei of G. duodenalis. The hammerhead ribozyme depleted ZFD mRNA levels, which reduced the reproduction rate of G. duodenalis, telomerase activity and telomere length.

conclusionsOur findings suggest that ZFD may regulate telomere function in G. duodenalis nuclei.

Indexed as

Gene Expression RegulationZinc FingersCell NucleusGiardia lambliaHEK293 CellsHumansImmunoprecipitationProtozoan ProteinsRNARNA, CatalyticTelomeraseTwo-Hybrid System Techniqueshammerhead ribozymeProtozoan ProteinsRNARNA, CatalyticTelomerasetelomerase RNAGiardia duodenalisProtein–protein interactionTelomerase

Identifiers

PMID31783771
PMCPMC6884763
OpenAlexW2989777789

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.