ArticleAging cell2020
Base excision repair but not DNA double-strand break repair is impaired in aged human adipose-derived stem cells.
Article in Aging cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- IGF2BP3-dependent glutamine/BCAA metabolic rewiring rejuvenates aged human adipose-derived stem cells for enhanced tissue regeneration.Cell discovery · 2026Article
- Roles of chromatin and genome instability in cellular senescence and their relevance to ageing and related diseases.Nature reviews. Molecular cell biology · 2024Review
- HES1 revitalizes the functionality of aged adipose-derived stem cells by inhibiting the transcription of STAT1.Stem cell research & therapy · 2024Article
- The transcription factor PAX5 activates human LINE1 retrotransposons to induce cellular senescence.EMBO reports · 2024Article
- The cGAS-Ku80 complex regulates the balance between two end joining subpathways.Cell death and differentiation · 2024Article
- The Rejuvenation and Functional Restoration of Aged Adipose Stem Cells by DUXAP10 Knockdown via the Regulation of the miR-214-3p/RASSF5 Axis.Stem cells translational medicine · 2024Article
- Androgen signaling stabilizes genomes to counteract senescence by promoting XRCC4 transcription.EMBO reports · 2023Article
- Biomarkers of aging.Science China. Life sciences · 2023Review
- The accelerated aging phenotype: The role of race and social determinants of health on aging.Ageing research reviews · 2022Review
- Is Adipose Tissue the Fountain of Youth? The Impact of Adipose Stem Cell Aging on Metabolic Homeostasis, Longevity, and Cell-Based Therapies.Advances in experimental medicine and biology · 2021Review
- The SIRT6 activator MDL-800 improves genomic stability and pluripotency of old murine-derived iPS cells.Aging cell · 2020Article
- Base excision repair but not DNA double-strand break repair is impaired in aged human adipose-derived stem cells.Aging cell · 2020Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
The decline in DNA repair capacity contributes to the age-associated decrease in genome integrity in somatic cells of different species. However, due to the lack of clinical samples and appropriate tools for studying DNA repair, whether and how age-associated changes in DNA repair result in a loss of genome integrity of human adult stem cells remains incompletely characterized. Here, we isolated 20 eyelid adipose-derived stem cell (ADSC) lines from healthy individuals (young: 10 donors with ages ranging 17-25 years; old: 10 donors with ages ranging 50-59 years). Using these cell lines, we systematically compared the efficiency of base excision repair (BER) and two DNA double-strand break (DSB) repair pathways-nonhomologous end joining (NHEJ) and homologous recombination (HR)-between the young and old groups. Surprisingly, we found that the efficiency of BER but not NHEJ or HR is impaired in aged human ADSCs, which is in contrast to previous findings that DSB repair declines with age in human fibroblasts. We also demonstrated that BER efficiency is negatively associated with tail moment, which reflects a loss of genome integrity in human ADSCs. Mechanistic studies indicated that at the protein level XRCC1, but not other BER factors, exhibited age-associated decline. Overexpression of XRCC1 reversed the decline of BER efficiency and genome integrity, indicating that XRCC1 is a potential therapeutic target for stabilizing genomes in aged ADSCs.
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Registered trials
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