Evidence map›Paper›PMID 31781817›Full record

ArticleApplied microbiology and biotechnology2020

Factors determining microbial colonization of liquid nitrogen storage tanks used for archiving biological samples.

F Bajerski, A Bürger, B Glasmacher, E R J Keller, K Müller, K Mühldorfer, M Nagel, H Rüdel, T Müller, J Schenkel and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Guideline
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 8 institutions in 1 country.

F BajerskiLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, Braunschweig, Germany. Felizitas.Bajerski@dsmz.de.ORCID http://orcid.org/0000-0002-9912-3654
A BürgerHelmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, München, Germany.
B GlasmacherInstitute for Multiphase Processes, Leibniz University Hannover, Hannover, Germany.
E R J KellerLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, OT Gatersleben, Germany.
K MüllerLeibniz Institute for Zoo and Wildlife Research (Leibniz-IZW), Berlin, Germany.
K MühldorferLeibniz Institute for Zoo and Wildlife Research (Leibniz-IZW), Berlin, Germany.
M NagelLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, OT Gatersleben, Germany.
H RüdelFraunhofer Institute for Molecular Biology and Applied Ecology IME, Schmallenberg, Germany.
T MüllerBioKryo GmbH, Sulzbach, Germany.
J SchenkelGerman Cancer Research Centre, Heidelberg, Germany.
J OvermannLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, Braunschweig, Germany.
Leibniz Institute for Zoo and Wildlife Research · DELeibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) · DEFraunhofer Institute for Molecular Biology and Applied Ecology · DEGerman Cancer Research Center · DEHeidelberg University · DEHelmholtz Zentrum München · DELeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures · DELeibniz University Hannover · DE

Funding

GDK Gemeinschaft Deutscher Kryobanken GDKHelmholtz-Gemeinschaft ExNet-0041-Phase2-3Helmholtz-Gemeinschaft FP7-HEALTH-F4-2010-261492
6 · The paper itself

Abstract

The availability of bioresources is a precondition for life science research, medical applications, and diagnostics, but requires a dedicated quality management to guarantee reliable and safe storage. Anecdotal reports of bacterial isolates and sample contamination indicate that organisms may persist in liquid nitrogen (LN) storage tanks. To evaluate the safety status of cryocollections, we systematically screened organisms in the LN phase and in ice layers covering inner surfaces of storage tanks maintained in different biobanking facilities. We applied a culture-independent approach combining cell detection by epifluorescence microscopy with the amplification of group-specific marker genes and high-throughput sequencing of bacterial ribosomal genes. In the LN phase, neither cells nor bacterial 16S rRNA gene copy numbers were detectable (detection limit, 10

Indexed as

Equipment ContaminationNitrogenBacteriaBacterial LoadBiological Specimen BanksCryopreservationDNA, BacterialFungiHumansIceLimit of DetectionRNA, Ribosomal, 16SDNA, BacterialIceNitrogenRNA, Ribosomal, 16SAmplicon sequencingBiobankingCryobankCryopreservationMicrobial contaminationRisk/quality managementSafe storage

Identifiers

PMID31781817
PMCPMC6942587
OpenAlexW2990988363

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.