ArticleJournal of experimental botany2020
Integrative analysis of the nuclear proteome in Pinus radiata reveals thermopriming coupled to epigenetic regulation.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Short-Term Epigenetic Responses ofGenes · 2026Article
- Epigenetics in forest trees- key driver to phenotypic plasticity and adaptation under stress.Functional & integrative genomics · 2025Review
- Pra-GE-ATLAS: Empowering Pinus radiata stress and breeding research through a multi-omics database.Journal of integrative plant biology · 2025Article
- Using targeted genome methylation for crop improvement.Journal of experimental botany · 2025Review
- Temperature-induced variation in the transcriptome of maritime pine (Pinus pinaster Ait.) embryogenic masses modulates the phenotype of the derived plants.BMC genomics · 2025Article
- Epigenetic responses of trees to environmental stress in the context of climate change.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Epigenetic stress memory in gymnosperms.Plant physiology · 2024Review
- Comprehensive Organ-Specific Profiling of Douglas Fir (Biomolecules · 2023Article
- Proteomic and Metabolic Analysis ofInternational journal of molecular sciences · 2023Article
- The Integration of Data from Different Long-Read Sequencing Platforms Enhances Proteoform Characterization in Arabidopsis.Plants (Basel, Switzerland) · 2023Article
- Revealing the transitory and local effect of zebularine on development and on proteome dynamics ofFrontiers in plant science · 2023Article
- Proteomics research in forest trees: A 2012-2022 update.Frontiers in plant science · 2023Review
- Integrative analysis in Pinus revealed long-term heat stress splicing memory.The Plant journal : for cell and molecular biology · 2022Article
- DNA methylation dynamics in response to abiotic and pathogen stress in plants.Plant cell reports · 2022Review
- DNA methylation and histone modifications induced by abiotic stressors in plants.Genes & genomics · 2022Review
- Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Can Forest Trees Cope with Climate Change?-Effects of DNA Methylation on Gene Expression and Adaptation to Environmental Change.International journal of molecular sciences · 2021Review
- Protein expression plasticity contributes to heat and drought tolerance of date palm.Oecologia · 2021Article
- Challenges and Perspectives in the Epigenetics of Climate Change-Induced Forests Decline.Frontiers in plant science · 2021Review
- Proteome-Wide Analysis of Heat-Stress inFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite it being an important issue in the context of climate change, for most plant species it is not currently known how abiotic stresses affect nuclear proteomes and mediate memory effects. This study examines how Pinus radiata nuclei respond, adapt, 'remember', and 'learn' from heat stress. Seedlings were heat-stressed at 45 °C for 10 d and then allowed to recover. Nuclear proteins were isolated and quantified by nLC-MS/MS, the dynamics of tissue DNA methylation were examined, and the potential acquired memory was analysed in recovered plants. In an additional experiment, the expression of key gene genes was also quantified. Specific nuclear heat-responsive proteins were identified, and their biological roles were evaluated using a systems biology approach. In addition to heat-shock proteins, several clusters involved in regulation processes were discovered, such as epigenomic-driven gene regulation, some transcription factors, and a variety of RNA-associated functions. Nuclei exhibited differential proteome profiles across the phases of the experiment, with histone H2A and methyl cycle enzymes in particular being accumulated in the recovery step. A thermopriming effect was possibly linked to H2A abundance and over-accumulation of spliceosome elements in recovered P. radiata plants. The results suggest that epigenetic mechanisms play a key role in heat-stress tolerance and priming mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.