ArticleScientific reports2019
Cell alignment and accumulation using acoustic nozzle for bioprinting.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Acoustic Tweezers-Assisted Biomaterial Molding in Petri Dishes (TAMP) for Engineering Glioma Tissues With Controlled Cellular and Extracellular Matrix Architectures.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Advances in extrusion-based bioprinting enabled by advanced printhead and nozzle designs.Materials today. Bio · 2026Review
- Unconventional bioprinting modalities for advanced tissue biofabrication.Biomaterials · 2026Review
- Acoustic Bioprinting: A Glimpse Into an Emerging Field.Small methods · 2026Review
- 3D Hybrid Bioprinting for Complex Multi-Tissue Engineering.bioRxiv : the preprint server for biology · 2025Article
- 3D printing of hydrogels: a synergistic approach of rheology and computational fluid dynamics (CFD) modeling.RSC advances · 2025Article
- 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation.Biofabrication · 2025Review
- Recent Advances in Handheld and Robotic Bioprinting Approach for Tissue Engineering.Advanced materials technologies · 2025Article
- Advances in Fibrin-Based Bioprinting for Skin Tissue Regeneration: Exploring Design, and Innovative Approaches.Biomedical materials & devices (New York, N.Y.) · 2025Article
- Droplet-based bioprinting.Nature reviews. Methods primers · 2025Article
- Progress in Organ Bioprinting for Regenerative Medicine.Engineering (Beijing, China) · 2024Article
- Review
- Diverse Applications of Three-Dimensional Printing in Biomedical Engineering: A Review.3D printing and additive manufacturing · 2023Review
- 3D bioprinted scaffolds for diabetic wound-healing applications.Drug delivery and translational research · 2023Article
- Manipulation with sound and vibration: A review on the micromanipulation system based on sub-MHz acoustic waves.Ultrasonics sonochemistry · 2023Review
- Review
- 3D printability and biochemical analysis of revalorized orange peel waste.International journal of bioprinting · 2023Article
- Extrusion 3D (Bio)Printing of Alginate-Gelatin-Based Composite Scaffolds for Skeletal Muscle Tissue Engineering.Materials (Basel, Switzerland) · 2022Review
- Myocardial infarction from a tissue engineering and regenerative medicine point of view: A comprehensive review on models and treatments.Biophysics reviews · 2022Review
- Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review.Gels (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioprinting could spatially align various cells in high accuracy to simulate complex and highly organized native tissues. However, the uniform suspension and low concentration of cells in the bioink and subsequently printed construct usually results in weak cell-cell interaction and slow proliferation. Acoustic manipulation of biological cells during the extrusion-based bioprinting by a specific structural vibration mode was proposed and evaluated. Both C2C12 cells and human umbilical vein endothelial cells (HUVECs) could be effectively and quickly accumulated at the center of the cylindrical tube and consequently the middle of the printed construct with acoustic excitation at the driving frequency of 871 kHz. The full width at half maximum (FWHM) of cell distributions fitted with a Gaussian curve showed a significant reduction by about 2.2 fold in the printed construct. The viability, morphology, and differentiation of these cells were monitored and compared. C2C12 cells that were undergone the acoustic excitation had nuclei oriented densely within ±30° and decreased circularity index by 1.91 fold or significant cell elongation in the printing direction. In addition, the formation of the capillary-like structure in the HUVECs construct was found. The number of nodes, junctions, meshes, and branches of HUVECs on day 14 was significantly greater with acoustic excitation for the enhanced neovascularization. Altogether, the proposed acoustic technology can satisfactorily accumulate/pattern biological cells in the printed construct at high biocompatibility. The enhanced cell interaction and differentiation could subsequently improve the performance and functionalities of the engineered tissue samples.
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