Evidence map›Paper›PMID 31780101›Full record

ArticleDental materials : official publication of the Academy of Dental Materials2020

Micropatterned hydrogels and cell alignment enhance the odontogenic potential of stem cells from apical papilla in-vitro.

Michael Ha, Avathamsa Athirasala, Anthony Tahayeri, Paula P Menezes, Luiz E Bertassoni

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Dental materials : official publication of the Academy of Dental Materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Cell homing within endodontic hydrogels: A scoping review.The Japanese dental science review · 2026
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  17. Advances on Hydrogels for Oral Science Research.Gels (Basel, Switzerland) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Michael HaDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA.
Avathamsa AthirasalaDepartment of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA.
Anthony TahayeriDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA.
Paula P MenezesDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA; Postgraduate Program in Health Sciences, Department of Pharmacy, Federal University of Sergipe, Aracaju, SE, Brazil.
Luiz E BertassoniDivision of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University, Portland, OR, USA; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA; Center for Regenerative Medicine, Oregon Health and Science University, Portland, OR, USA; Cancer Early Detection Advanced Research, Knight Cancer Institute, Portland, OR, USA. Electronic address: bertasso@ohsu.edu.

Funding

Microengineering the Dental Pulp Vascular Microenvironment_Diversity SupplementR01DE026170 · NIDCR · OREGON HEALTH & SCIENCE UNIVERSITY · PI BERTASSONI, LUIZ EDUARDO · 2016 to 2020
$2.5M
NIDCR NIH HHS R01 DE026170
6 · The paper itself

Abstract

introductionAn understanding of the extracellular matrix characteristics which stimulate and guide stem cell differentiation in the dental pulp is fundamental for the development of enhanced dental regenerative therapies. Our objectives, in this study, were to determine whether stem cells from the apical papilla (SCAP) responded to substrate stiffness, whether hydrogels providing micropatterned topographical cues stimulate SCAP self-alignment, and whether the resulting alignment could influence their differentiation towards an odontogenic lineage in-vitro.

methodsExperiments utilized gelatin methacryloyl (GelMA) hydrogels of increasing concentrations (5, 10 and 15%). We determined their compressive modulus via unconfined compression and analyzed cell spreading via F-actin/DAPI immunostaining. GelMA hydrogels were micropatterned using photolithography, in order to generate microgrooves and ridges of 60 and 120μm, onto which SCAP were seeded and analyzed for self-alignment via fluorescence microscopy. Lastly, we analyzed the odontogenic differentiation of SCAP using alkaline phosphatase protein expression (ANOVA/Tukey α=0.05).

resultsSCAP appeared to proliferate better on stiffer hydrogels. Both 60 and 120μm micropatterned hydrogels guided the self-alignment of SCAP with no significant difference between them. Similarly, both 60 and 120μm micropattern aligned cells promoted higher odontogenic differentiation than non-patterned controls. SIGNIFICANCE: In summary, both substrate mechanics and geometry have a statistically significant influence on SCAP response, and may assist in the odontogenic differentiation of dental stem cells. These results may point toward the fabrication of cell-guiding scaffolds for regenerative endodontics, and may provide cues regarding the development of the pulp-dentin interface during tooth formation.

Indexed as

HydrogelsOdontogenesisCell DifferentiationCell ProliferationDental PapillaDental PulpStem CellsHydrogelsGelMAMechanotransductionMicropatterningOdontogenic differentiationRegenerative dentistry

Identifiers

PMID31780101
PMCPMC7395926

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.