ArticleJournal of leukocyte biology2020
Proliferation of Ly6C+ monocytes/macrophages contributes to their accumulation in mouse skin wounds.
Article in Journal of leukocyte biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- A cell differentiation landscape for monocyte and interstitial macrophage in the lung with diffuse alveolar damage.Protein & cell · 2026Article
- Effect of ProbioticThe Malaysian journal of medical sciences : MJMS · 2025Article
- Mechanosensitivity of macrophage polarization: comparing small molecule leukadherin-1 to substrate stiffness.Frontiers in immunology · 2025Article
- Conserved and tissue-specific immune responses to biologic scaffold implantation.Acta biomaterialia · 2024Article
- Spatiotemporal orchestration of macrophage activation trajectories by Vγ4 T cells during skin wound healing.iScience · 2024Article
- Diet-Induced Obesity Increases Monocyte/Macrophage Proliferation during Skin Wound Healing in Mice.Cells · 2024Article
- A bioelectronic device for electric field treatment of wounds reduces inflammation in an in vivo mouse model.PloS one · 2024Article
- Proliferation of monocytes and macrophages in homeostasis, infection, injury, and disease.Journal of leukocyte biology · 2023Review
- Conserved and tissue-specific immune responses to biologic scaffold implantation.bioRxiv : the preprint server for biology · 2023Article
- Advances in Zebrafish for Diabetes Mellitus with Wound Model.Bioengineering (Basel, Switzerland) · 2023Review
- Behind the monocyte's mystique: uncovering their developmental trajectories and fates.Discovery immunology · 2023Review
- Monocyte/Macrophage Heterogeneity during Skin Wound Healing in Mice.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Transitional premonocytes emerge in the periphery for host defense against bacterial infections.Science advances · 2022Article
- Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis.Frontiers in immunology · 2022Article
- Macrophage-mediated inflammation in diabetic wound repair.Seminars in cell & developmental biology · 2021Review
- Skin Wound Healing: Normal Macrophage Function and Macrophage Dysfunction in Diabetic Wounds.Molecules (Basel, Switzerland) · 2021Review
- Reduced apoptosis of monocytes and macrophages is associated with their persistence in wounds of diabetic mice.Cytokine · 2021Article
- Enhanced Proliferation of Ly6CJournal of immunology (Baltimore, Md. : 1950) · 2021Article
- Macrophages in Healing Wounds: Paradoxes and Paradigms.International journal of molecular sciences · 2021Review
- Macrophage Dysregulation and Impaired Skin Wound Healing in Diabetes.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Monocytes and macrophages (Mo/MΦ) play critical roles in all phases of skin wound healing. The majority of these cells are thought to be recruited from blood Mo; however, the role local proliferation of Mo/MΦ in the wound has not been defined. Therefore, we tested the hypothesis that local proliferation of Mo and/or MΦ contributes to their accumulation during wound healing. Male C57Bl/6 mice (N = 4-9/group) were subjected to excisional skin wounding. Proliferating Mo/MΦ (F4/80+Ki67+) were observed in wound cryosections, peaking on day 5 post-wounding. Cell cycle analysis on cells isolated from skin tissue revealed that wounding increased both the number and percentage of inflammatory Ly6C+F4/80lo/- Mo/MΦ in the S/G2/M phases, peaking on day 6 post-wounding. In contrast, more mature Ly6C-F4/80+ cells were found predominantly in the G0 phase with less than 1% cells in S/G2/M phase following injury. In peripheral blood, Mo were very rarely found in the S/G2/M phase, suggesting that the wound environment triggered the Ly6C+F4/80lo/- Mo proliferative response. Furthermore, injury induced several potential regulators of proliferation in wounds, including IL-1β and IL-6, and wound Mo/MΦ expressed surface receptors for these cytokines. However, wound Mo/MΦ proliferation was not altered in IL-1R1 knockout (KO) or IL-6 KO mice. In summary, our findings indicate that proliferation contributes to Mo/MΦ accumulation in wounds and, contrary to findings in other pathophysiologic conditions, Ly6C+/F4/80lo/- Mo/MΦ proliferate during skin wound healing whereas mature Ly6C-F4/80+ MΦ do not.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.