ArticleAnnals of botany2020
Assessing radiation dose limits for X-ray fluorescence microscopy analysis of plant specimens.
Article in Annals of botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- The nature of thallium crystals in Brassica oleracea (kale): a synchrotron multi-technique investigation.Metallomics : integrated biometal science · 2026Article
- X-ray micro-CT imaging to study foliar water uptake mechanisms in plants with contrasting leaf topography.The New phytologist · 2025Article
- Synchrotron X-ray fluorescence microscopy unveils selenium distribution and a phloem-sink hypothesis in Neptunia amplexicaulis.Plant physiology · 2025Article
- Extraction, Characterization, Biological Properties, and X-Ray Fluorescence Analysis of Functional Polysaccharides Derived fromLife (Basel, Switzerland) · 2025Article
- Correlative X-ray imaging to reveal the dissolution of nanoparticles and nutrient transport in plant foliar fertilization.Frontiers in plant science · 2025Article
- The curious case of selenium hyperaccumulation in Coelospermum decipiens from the Cape York Peninsula (Queensland, Australia).Annals of botany · 2024Article
- Research progress of the detection and analysis methods of heavy metals in plants.Frontiers in plant science · 2024Review
- High-energy interference-free K-lines synchrotron X-ray fluorescence microscopy of rare earth elements in hyperaccumulator plants.Metallomics : integrated biometal science · 2023Article
- Chemical transformations of arsenic in the rhizosphere-root interface of Pityrogramma calomelanos and Pteris vittata.Metallomics : integrated biometal science · 2023Article
- Fast X-ray fluorescence microscopy provides high-throughput phenotyping of element distribution in seeds.Plant physiology · 2023Article
- Synchrotron XFM tomography for elucidating metals and metalloids in hyperaccumulator plants.Metallomics : integrated biometal science · 2022Article
- Cellular-level distribution of manganese in Macadamia integrifolia, M. ternifolia, and M. tetraphylla from Australia.Metallomics : integrated biometal science · 2022Article
- X-ray fluorescence microscopy methods for biological tissues.Metallomics : integrated biometal science · 2022Review
- Time-resolved laboratory micro-X-ray fluorescence reveals silicon distribution in relation to manganese toxicity in soybean and sunflower.Annals of botany · 2020Article
- Methods to Visualize Elements in Plants.Plant physiology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsX-ray fluorescence microscopy (XFM) is a powerful technique to elucidate the distribution of elements within plants. However, accumulated radiation exposure during analysis can lead to structural damage and experimental artefacts including elemental redistribution. To date, acceptable dose limits have not been systematically established for hydrated plant specimens.
methodsHere we systematically explore acceptable dose rate limits for investigating fresh sunflower (Helianthus annuus) leaf and root samples and investigate the time-dose damage in leaves attached to live plants. KEY
resultsWe find that dose limits in fresh roots and leaves are comparatively low (4.1 kGy), based on localized disintegration of structures and element-specific redistribution. In contrast, frozen-hydrated samples did not incur any apparent damage even at doses as high as 587 kGy. Furthermore, we find that for living plants subjected to XFM measurement in vivo and grown for a further 9 d before being reimaged with XFM, the leaves display elemental redistribution at doses as low as 0.9 kGy and they continue to develop bleaching and necrosis in the days after exposure.
conclusionsThe suggested radiation dose limits for studies using XFM to examine plants are important for the increasing number of plant scientists undertaking multidimensional measurements such as tomography and repeated imaging using XFM.
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