ArticleJournal of assisted reproduction and genetics2020
A novel "embryo-endometrial" adhesion model can potentially predict "receptive" or "non-receptive" endometrium.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- MiR-29c-3p Impairs the Adhesion of Endometrial Epithelial Cells via COL4A1/β-catenin in Endometriosis.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Article
- Engineering the Human Endometrial-Embryo Interface: Breakthroughs in 3D Uterine Models.Biomolecules · 2026Review
- Back to the Future-A 50-Year Dive into Embryo Implantation Research: Cell Biological Paradox, Epithelial Cell Polarity, and EMT.Biomolecules · 2026Review
- Modeling Early Human Embryo Implantation Using Human Endometrial Organoids and Embryo Surrogate Co-cultures for microRNA Transfection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bioprinted Hormone-Responsive Bilayer Model of Human Endometrium for Embryo Implantation Studies.ACS biomaterials science & engineering · 2025Article
- Establishment of an in vitro implantation model using a newly developed mouse endometrial organoid.Development (Cambridge, England) · 2025Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Upregulated α-actinin-1 impairs endometrial epithelial cell adhesion by downregulating NEBL in recurrent implantation failure.iScience · 2024Article
- Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness.Bioactive materials · 2023Article
- Secretory Proteomic Responses of Endometrial Epithelial Cells to Trophoblast-Derived Extracellular Vesicles.International journal of molecular sciences · 2023Article
- The effect of obesity on uterine receptivity is mediated by endometrial extracellular vesicles that control human endometrial stromal cell decidualization and trophoblast invasion.Journal of extracellular biology · 2023Article
- Increased expression of HMGB1 in the implantation phase endometrium is related to recurrent implantation failure.Molecular biology reports · 2022Article
- Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape.Frontiers in cell and developmental biology · 2022Article
- Organoids to model the endometrium: implantation and beyond.Reproduction & fertility · 2021Review
- In vitro models of the human endometrium: evolution and application for women's health.Biology of reproduction · 2021Review
- Novel 3D embryo implantation model within macroporous alginate scaffolds.Journal of biological engineering · 2020Article
- Establishment of a Novel Human Endometrial Organoid.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo establish a model of human implantation that responds to hormonal stimuli and can differentiate between endometrium from fertile women and those with idiopathic infertility.
designA trophoblast stem cell (trophectodermal) line (TSC; derived from human pre-implantation embryo) was used to form trophectodermal spheroids (TS). TS attachment to monolayers of endometrial epithelial cell lines or primary endometrial epithelial cells (pHEECs) was determined.
settingIndependent Medical Research Institute with close clinical linkages
interventionsSpheroid attachment and outgrowth was determined with added hormones (estradiol 17β (E), E + medroxyprogesterone acetate (MPA) or E + MPA + human chorionic gonadotropin (hCG)). Spheroid attachment to E/MPA treated pHEEC prepared from fertile women or those with idiopathic infertility tested.
main outcome measureFirmly attached spheroids counted after co-culture for 6 h. Outgrowth was determined by quantitation of area covered by spheroid after firm adhesion.
resultsFunctional adhesion of TS to two endometrial epithelial cell lines, Ishikawa and ECC-1 cells, was hormonally responsive, with adhesion/outgrowth increased by E/MPA (ECC-1; p < 0.01, Ishikawa; p < 0.01) and E/MPA/hCG (ECC-1; p < 0.001, Ishikawa p < 0.01) versus E alone. The same pattern of hormone responsiveness was observed in pHEEC obtained from fertile women (E vs, E/MPA; p < 0.01, E vs. E/MPA/hCG; p < 0.001). TS adhered to 85% of pHEEC obtained from fertile women (11/13) and 11% of pHEEC obtained from women with unexplained infertility (2/18, p < 0.001).
conclusionThis new model of "embryo" implantation largely discriminates between endometrial epithelial cells obtained from fertile vs. infertile women based on adhesion; this holds potential as an in vitro "diagnostic" tool of endometrial infertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.