Evidence map›Paper›PMID 31776681›Full record

ArticleMikrochimica acta2019

Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides.

Dilshad Hussain, Syed Ghulam Musharraf, Batool Fatima, Adeela Saeed, Fahmida Jabeen, Muhammad Naeem Ashiq, Muhammad Najam-Ul-Haq

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Modification of Luffa Sponge for Enrichment of Phosphopeptides.International journal of molecular sciences · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Dilshad HussainDivision of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60000, Pakistan.
Syed Ghulam MusharrafHEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Batool FatimaDepartment of Biochemistry, Bahauddin Zakariya University, Multan, 60000, Pakistan.
Adeela SaeedDepartment of Chemistry, The Women University, Multan, 60000, Pakistan.
Fahmida JabeenDepartment of Chemistry, The Women University, Multan, 60000, Pakistan.
Muhammad Naeem AshiqDivision of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60000, Pakistan.
Muhammad Najam-Ul-HaqDivision of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60000, Pakistan. najamulhaq@bzu.edu.pk.ORCID 0000-0002-2090-5072
Bahauddin Zakariya University · PKThe Women University Multan · PKUniversity of Karachi · PK

Funding

Higher Education Commission (HEC) Pakistan NIL
6 · The paper itself

Abstract

An anion exchange solid-phase sorbent is described. Chitosan coated magnetite nanoparticles were modified with polyethylenimine which is positively charged at pH 3 and therefore can be used for the magnet-supported enrichment of phosphopeptides which are negatively charged at this pH value. A 2-step strategy was used to synthesize the sorbent. The materials were characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry and magnetic moment analysis. The anion exchanger was applied to extract phosphopeptides from a β-casein digest. Characteristic analytical figures include (a) a loading buffer of pH 3, (b) and elution buffer of pH 11, (c) a loading time of 5 min, (d) good selectivity (the β-casein to BSA ratio is 1:1000), and (e) excellent sensitivity (1 fmol). The optimized method was applied to egg yolk digest, non-fat milk digest, and diluted human serum. Graphical abstractSchematic representation of synthesis of PEI@chitosan@Fe

Indexed as

AdsorptionAnimalsAnionsCattleChitosanEgg YolkHumansMagnetite NanoparticlesMilkParticle SizePhosphopeptidesPolyethyleneimineSurface PropertiesAnionsChitosanMagnetite NanoparticlesPhosphopeptidesPolyethyleneimineAffinity materialAnion exchangerEgg-yolkEndogenous phosphopeptidesHuman serumMagnetic iron oxide nanoparticlesNon-fat milkSelectivity

Identifiers

PMID31776681
OpenAlexW2991328362

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.