Evidence map›Paper›PMID 31776666›Full record

ArticlePlanta2019

Exosomes in the phloem and xylem of woody plants.

Dmitry G Chukhchin, Ksenia Bolotova, Igor Sinelnikov, Dmitry Churilov, Evgeniy Novozhilov

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
5.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 44 citations in OpenAlex.

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  20. Characterization of Oligopeptides inLife (Basel, Switzerland) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Dmitry G ChukhchinNorthern (Arctic) Federal University, Northern Dvina Embankment 17, 163000, Arkhangelsk, Russia.
Ksenia BolotovaNorthern (Arctic) Federal University, Northern Dvina Embankment 17, 163000, Arkhangelsk, Russia.
Igor SinelnikovFederal State Institution "Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences", Leninsky Prospect, 33, Build. 2, 119071, Moscow, Russian Federation.
Dmitry ChurilovNorthern (Arctic) Federal University, Northern Dvina Embankment 17, 163000, Arkhangelsk, Russia.
Evgeniy NovozhilovNorthern (Arctic) Federal University, Northern Dvina Embankment 17, 163000, Arkhangelsk, Russia. e.novozhilov@narfu.ru.
Northern (Arctic) Federal University · RUFederal Center Research Fundamentals of Biotechnology · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MAIN

conclusionExosomes in the secondary phloem and secondary xylem of angiosperms and gymnosperms have physiological roles in the storage and transport of endoglucanases. Knowledge of plant extracellular vesicles (EVs) is limited by their presence in the apoplastic fluid of seeds and leaves. The contents of plant EVs and their biological functions are unclear. The aim of the present study was to expand our knowledge of EVs in woody plants. Sample splits were prepared from branch and stem samples from angiosperms and gymnosperms after cryomechanical destruction with liquid nitrogen. The study methods included scanning electron (SEM), atomic force microscopy (AFM), endoglucanase activity measurement. EVs visualized on the internal layers of the cell walls proved to be exosomes according to their diameter (65-145 nm). SEM revealed cup-shaped structures characteristic of exosomes in a dry state. Plant exosomes in the form of globules in the native state were visualized for the first time by AFM. Exosomes were present both in the active and dormant cambium. Erosion zones were observed at the sites of exosome localization. The activity of endo-1,4-β-glucanase was detected in Picea xylem, while the RNA level was very low, suggesting that endo-1,4-β-glucanases were preserved in the exosomes. There are grounds to assert that endo-1,4-β-glucanases delivered by exosomes participated in pit cavity formation in the S

Indexed as

CellulaseExosomesGene Expression Regulation, PlantMicroscopy, Electron, ScanningPhloemXylemCellulaseCell wall remodelingCryomechanical destructionEndo-1,4-β-glucanaseExtracellular vesiclesPit formationScanning electron microscopy

Identifiers

PMID31776666
OpenAlexW2990688408

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.